ArticlePlant physiology2022
Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 14 citations in OpenAlex.
- Two pattern recognition receptors provide quantitative resistance to Gray Leaf Spot in Maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- CRISPR/Cas genome editing in soybean: challenges and new insights to overcome existing bottlenecks.Journal of advanced research · 2025Review
- The speed breeding technology of five generations per year in cotton.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Development of efficient targeted insertion mediated by CRISPR-Cas12a and homology-directed repair in maize.Frontiers in genome editing · 2025Article
- Article
- Identification of Ochrobactrum as a bacteria with transstadial transmission and potential for application in paratransgenic control of leishmaniasis.Parasitology research · 2024Article
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- CRISPR/LbCas12a-Mediated Genome Editing in Soybean.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Plant breeding advancements with "CRISPR-Cas" genome editing technologies will assist future food security.Frontiers in plant science · 2023Review
- Updates on gene editing and its applications.Plant physiology · 2022Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
- Enhancing HR Frequency for Precise Genome Editing in Plants.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene targeting (GT) for precise gene insertion or swap into pre-defined genomic location has been a bottleneck for expedited soybean precision breeding. We report a robust selectable marker-free GT system in soybean, one of the most economically important crops. An efficient Oh H1-8 (Ochrobactrum haywardense H1-8)-mediated embryonic axis transformation method was used for the delivery of CRISPR-Cas9 components and donor template to regenerate T0 plants 6-8 weeks after transformation. This approach generated up to 3.4% targeted insertion of the donor sequence into the target locus in T0 plants, with ∼ 90% mutation rate observed at the genomic target site. The GT was demonstrated in two genomic sites using two different donor DNA templates without the need for a selectable marker within the template. High-resolution Southern-by-Sequencing analysis identified T1 plants with precise targeted insertion and without unintended plasmid DNA. Unlike previous low-frequency GT reports in soybean that involved particle bombardment-mediated delivery and extensive selection, the method described here is fast, efficient, reproducible, does not require a selectable marker within the donor DNA, and generates nonchimeric plants with heritable GT.
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