ArticleNature biotechnology2022
Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale.
Article in Nature biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers.
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153 citing papers in PubMed.
- Thermodynamic metabolic modeling of growth and bioproduction potential of the acetogenSynthetic and systems biotechnology · 2026Article
- Reprogramming energy system for growth and bioproduction from CO₂ and methanol in Escherichia coli.Nature communications · 2026Article
- Continuous autotrophic production of alcohols with Clostridium autoethanogenum LAbrini in a submerged membrane bioreactor.Bioresources and bioprocessing · 2026Article
- The potential of syngas as a central mediator for a carbon-agnostic circular economy.Nature communications · 2026Review
- Environmental Impact of Synthetic Cell Technology: Review of Life Cycle Assessment Data for Feedstocks and Production Methods.Chembiochem : a European journal of chemical biology · 2026Review
- A characterized promoter suite enables metabolic rewiring of the acetogen Acetobacterium woodii toward ethanol production.Biotechnology for biofuels and bioproducts · 2026Article
- Systems-level analysis predicts no autotrophy-linked protein-RNA interactions in Clostridium autoethanogenum.Nature communications · 2026Article
- Zeolite membrane reactors for efficient catalytic conversion of CONational science review · 2026Review
- SynBio-DSP: an integrated synthetic biology platform with fed-batch fermentation and continuous downstream processing for sustainable production of thermostable cellulases.Biotechnology for biofuels and bioproducts · 2026Article
- Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system.Nature microbiology · 2026Article
- Valine overproduction inMetabolic engineering communications · 2026Article
- Solvent dehydration with structurally engineered nanoporous graphene oxide membranes.Nature communications · 2026Article
- Contribution of C1 Biotechnology to the Achievement of the United Nations' Sustainable Development Goals.Bioengineering (Basel, Switzerland) · 2026Review
- Designing Microbe-Semiconductor Interfaces for Semibiological Photosynthesis.Chemical reviews · 2026Review
- Dynamics of genetic circuits in Pseudomonas protegens.Cell systems · 2026Article
- Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expression.Nature communications · 2026Article
- Chemical Signaling and Metabolomic Crosstalk in Endophytic Fungi-Medicinal Plant Symbioses for Natural Product Discovery and Sustainable Bioproduction.Metabolites · 2026Review
- Optimizing Lactiplantibacillus plantarum JCM 1149 growth conditions for improvement of the gelation and nutritional qualities of fermented soy milk.Biotechnology letters · 2026Article
- Coupling Nitrogenous Organic Wastewater Treatment and Biorefinery via N-Cycling Bacterium.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Bioadaptive Ni single atoms unlock high rate microbial electrosynthesis of isopropanol from CONature communications · 2026Article
93 more citing papers are in PubMed but not listed here.
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Authors and funding
27 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many industrial chemicals that are produced from fossil resources could be manufactured more sustainably through fermentation. Here we describe the development of a carbon-negative fermentation route to producing the industrially important chemicals acetone and isopropanol from abundant, low-cost waste gas feedstocks, such as industrial emissions and syngas. Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum. Next, we used omics analysis, kinetic modeling and cell-free prototyping to optimize flux. Finally, we scaled-up our optimized strains for continuous production at rates of up to ~3 g/L/h and ~90% selectivity. Life cycle analysis confirmed a negative carbon footprint for the products. Unlike traditional production processes, which result in release of greenhouse gases, our process fixes carbon. These results show that engineered acetogens enable sustainable, high-efficiency, high-selectivity chemicals production. We expect that our approach can be readily adapted to a wide range of commodity chemicals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.