ArticleAnalytica chimica acta2022
Analyte recovery in LC-MS/MS bioanalysis: An old issue revisited.
Article in Analytica chimica acta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
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- Cationic covalent organic frameworks for simultaneous capture and high-performance enrichment of multiple angiotensins and aldosterone in human plasma.Mikrochimica acta · 2026Article
- Targeted Livestock Metabolomics: A Review of Liquid chromatography-Mass Spectrometry-Based Approaches.Journal of agricultural and food chemistry · 2026Review
- Machine learning prediction and calibration of cellulose-based solid-phase extraction performance for pharmaceuticals across aqueous matrices.RSC advances · 2026Review
- Comparative evaluation of immunoaffinity and antibody-free isotope dilution mass spectrometry approaches for plasma ProGRP quantification.Analytical and bioanalytical chemistry · 2026Article
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- Fractional Coprecipitation of Drugs and Natural Extracts with Zinc Hydroxide.Molecules (Basel, Switzerland) · 2025Article
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- Development and Validation of Targeted Metabolomics Methods Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) for the Quantification of 235 Plasma Metabolites.Molecules (Basel, Switzerland) · 2025Article
- Protein Precipitation by Metal Hydroxides as a Convenient and Alternative Sample Preparation Procedure for Bioanalysis.Molecules (Basel, Switzerland) · 2024Article
- Liquid chromatography-mass spectrometric method for the simultaneous analysis of branched-chain amino acids and their ketoacids from dried blood spot as secondary analytes for the detection of maple syrup urine disease.Journal of mass spectrometry and advances in the clinical lab · 2024Article
- Quantification of treprostinil concentration in rat and human using a novel validated and rapid liquid chromatography-tandem mass spectrometry method: Experimental and clinical applications in ischemia-reperfusion injury.Clinica chimica acta; international journal of clinical chemistry · 2024Article
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3 authors.
Funding
Abstract
There are several challenges associated with LC-MS/MS bioanalytical method development and validation. Low and variable recovery of some analytes, especially the more hydrophobic ones, is often challenging. Analytes can be lost to various extents throughout the process of sample collection, storage, before, during, and/or after sample preparation and analysis. The calculation of overall extraction recovery can detect problems of low recovery during sample preparation but does not identify the source(s) of analyte losses. Low overall analyte recovery is the net result of losses that can happen for multiple reasons at all steps of sample preparation and analysis. Therefore, identifying the source(s) of analyte loss during sample preparation can help guide the optimization the bioanalysis conditions to minimize these losses. In this article we propose a practical protocol to systematically identify and quantify the sources of low analyte recovery. This allows the proper choice of strategies to optimize the relevant bioanalytical conditions to minimize analyte losses and improve overall recovery.
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