ArticleCartilage
Engineering of Tracheal Grafts Based on Recellularization of Laser-Engraved Human Airway Cartilage Substrates.
Article in Cartilage. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Synergistic Effects of Non-Ionizing Radiation in the Targeted Modification of Living Tissues.International journal of molecular sciences · 2025Review
- Decellularized extracellular matrix-loaded exosome hydrogel for cell-free tracheal scaffold in tracheal defect reconstruction and repair.Journal of nanobiotechnology · 2025Article
- Decellularization techniques: unveiling the blueprint for tracheal tissue engineering.Frontiers in bioengineering and biotechnology · 2025Review
- Femtosecond Laser Engraving Promotes the Repopulation of Decellularized Human Articular Cartilage.Journal of tissue engineering and regenerative medicine · 2025Article
- Uterine Biosynthesis through Tissue Engineering: An Overview of Current Methods and Status.Current pharmaceutical biotechnology · 2025Review
- Pathologically altered articular cartilage attracts intense chondrocyte invasion into the extracellular matrix: in vitro pilot study.Knee surgery & related research · 2024Article
- Cartilage Laser Engraving for Fast-Track Tissue Engineering of Auricular Grafts.International journal of molecular sciences · 2024Article
- Stem Cells in Bone Tissue Engineering: Progress, Promises and Challenges.Stem cell reviews and reports · 2024Review
- Current Strategies for Tracheal Decellularization: A Systematic Review.International journal of biomaterials · 2024Review
- Post-Implantation Inflammatory Responses to Xenogeneic Tissue-Engineered Cartilage Implanted in Rabbit Trachea: The Role of Cultured Chondrocytes in the Modification of Inflammation.International journal of molecular sciences · 2023Article
- A Cartilaginous Construct with Bone Collar Exerts Bone-Regenerative Property Via Rapid Endochondral Ossification.Stem cell reviews and reports · 2023Article
- Distribution and chemotactic mechanism of CD4Immunity, inflammation and disease · 2023Article
- Anatomical Tissue Engineering of the Anterior Cruciate Ligament Entheses.International journal of molecular sciences · 2023Review
- Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ.Bioengineering (Basel, Switzerland) · 2022Review
- Preclinical and clinical orthotopic transplantation of decellularized/engineered tracheal scaffolds: A systematic literature review.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveImplantation of tissue-engineered tracheal grafts represents a visionary strategy for the reconstruction of tracheal wall defects after resections and may develop into a last chance for a number of patients with severe cicatricial stenosis. The use of a decellularized tracheal substrate would offer an ideally stiff graft, but the matrix density would challenge efficient remodeling into a living cartilage. In this study, we hypothesized that the pores of decellularized laser-perforated tracheal cartilage (LPTC) tissues can be colonized by adult nasal chondrocytes (NCs) to produce new cartilage tissue suitable for the repair of tracheal defects.
designHuman, native tracheal specimens, isolated from cadaveric donors, were exposed to decellularized and laser engraving-controlled superficial perforation (300 μm depth). Human or rabbit NCs were cultured on the LPTCs for 1 week. The resulting revitalized tissues were implanted ectopically in nude mice or orthotopically in tracheal wall defects in rabbits. Tissues were assayed histologically and by microtomography analyses before and after implantation.
resultsNCs were able to efficiently colonize the pores of the LPTCs. The extent of colonization (i.e., percentage of viable cells spanning >300 μm of tissue depth), cell morphology, and cartilage matrix deposition improved once the revitalized constructs were implanted ectopically in nude mice. LPTCs could be successfully grafted onto the tracheal wall of rabbits without any evidence of dislocation or tracheal stenosis, 8 weeks after implantation. Rabbit NCs, within the LPTCs, actively produced new cartilage matrix.
conclusionImplantation of NC-revitalized LPTCs represents a feasible strategy for the repair of tracheal wall defects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.