ArticlemBio2022
Polymicrobial Aggregates in Human Saliva Build the Oral Biofilm.
Article in mBio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Targeting of Oral Bacterial Taxa Linked to Disease by Flavonols.Nutrients · 2026Article
- An Innovative Oral Ex Vivo Biofilm Model for Antimicrobial Investigations.Pathogens (Basel, Switzerland) · 2026Article
- Nutritional pressure from serum amplifies dysbiotic features in an oral microbiome synthetic community.The ISME journal · 2026Article
- Antibacterial properties of polydopamine-modified ZnO nanoparticles composite films for oral therapeutic applications.Discover nano · 2026Article
- Review
- The Impact of Serum on a Complex Synthetic Community Model of the Subgingival Microbiome.bioRxiv : the preprint server for biology · 2025Article
- Biofilm development ofMicrobiology spectrum · 2025Article
- Article
- Characterization and ex vivo modelling of endodontic infections from the Arabian Gulf region.International endodontic journal · 2025Article
- Assessing the safety of microbiome perturbations.Microbial genomics · 2025Review
- Exploring the role of oral bacteria in oral cancer: a narrative review.Discover oncology · 2025Review
- Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols.Scientific reports · 2025Article
- The Oral Microbiome: A Key Determinant of Oral Health.Advances in experimental medicine and biology · 2025Review
- Alterations of the oral microbiota in mild Alzheimer's disease and the appropriate application of chlorhexidine gluconate.JAR life · 2025Article
- Formation, architecture, and persistence of oral biofilms: recent scientific discoveries and new strategies for their regulation.Frontiers in microbiology · 2025Review
- Coaggregation of oral pathogens by postbiotic lactobacilli.Journal of oral microbiology · 2025Article
- Acquisition of the Oral Microbiome.Advances in experimental medicine and biology · 2025Review
- Structure and composition of early biofilms formed on dental implants are complex, diverse, subject-specific and dynamic.NPJ biofilms and microbiomes · 2024Article
- Adhesive interactions within microbial consortia can be differentiated at the single-cell level through expansion microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 8 institutions in 3 countries.
Funding
Abstract
Biofilm community development has been established as a sequential process starting from the attachment of single cells on a surface. However, microorganisms are often found as aggregates in the environment and in biological fluids. Here, we conduct a comprehensive analysis of the native structure and composition of aggregated microbial assemblages in human saliva and investigate their spatiotemporal attachment and biofilm community development. Using multiscale imaging, cell sorting, and computational approaches combined with sequencing analysis, a diverse mixture of aggregates varying in size, structure, and microbial composition, including bacteria associated with host epithelial cells, can be found in saliva in addition to a few single-cell forms. Phylogenetic analysis reveals a mixture of complex consortia of aerobes and anaerobes in which bacteria traditionally considered early and late colonizers are found mixed together. When individually tracked during colonization and biofilm initiation, aggregates rapidly proliferate and expand tridimensionally, modulating population growth, spatial organization, and community scaffolding. In contrast, most single cells remain static or are incorporated by actively growing aggregates. These results suggest an alternative biofilm development process whereby aggregates containing different species or associated with human cells collectively adhere to the surface as "growth nuclei" to build the biofilm and shape polymicrobial communities at various spatial and taxonomic scales.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.