ArticleInternational journal of bioprinting2022
Controlling Droplet Impact Velocity and Droplet Volume: Key Factors to Achieving High Cell Viability in Sub-Nanoliter Droplet-based Bioprinting.
Article in International journal of bioprinting, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- 3D bioprinting of tissues and organs for systemic diseases and localized injuries.Military Medical Research · 2026Review
- Computational approaches in bioprinting processes.Nature reviews bioengineering · 2026Article
- 3D bioprinting of microfluidic systems for cardiac regenerative medicine: from biofabrication to organ-on-a-chip.Journal of biological engineering · 2026Review
- Three-dimensional bioprinted organoids: advances and clinical translation.Regenerative biomaterials · 2026Review
- An Overview of 3D Bioprinting Impact on Cell Viability: From Damage Assessment to Protection Solutions.Journal of functional biomaterials · 2025Review
- Impact dynamics of non-ferro liquid droplets under magnetic influence.RSC advances · 2025Article
- 3D Printing of Conducting Polymer Hydrogels for Electrostimulation-Assisted Tissue Engineering.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Review
- Visualization and quantification of local concentration gradients in evaporating water/glycerol droplets with micrometer resolution.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Machine Learning-Enhanced Optimization for High-Throughput Precision in Cellular Droplet Bioprinting.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Advancements in 3D skin bioprinting: processes, bioinks, applications and sensor integration.International journal of extreme manufacturing · 2025Review
- Sacrificial Templating for Accelerating Clinical Translation of Engineered Organs.ACS biomaterials science & engineering · 2025Review
- Recapitulating the bone extracellular matrix through 3D bioprinting using various crosslinking chemistries.Frontiers in bioengineering and biotechnology · 2025Review
- Understanding the cellular dynamics, engineering perspectives and translation prospects in bioprinting epithelial tissues.Bioactive materials · 2025Review
- Droplet-based bioprinting.Nature reviews. Methods primers · 2025Article
- Leveraging printability and biocompatibility in materials for printing implantable vessel scaffolds.Materials today. Bio · 2024Review
- Overview of research on additive manufacturing of hydrogel-assisted lab-on-chip platforms for cell engineering applications in photodynamic therapy research.Mikrochimica acta · 2024Review
- Dual Crosslinked Antioxidant Mixture of Poly(vinyl alcohol) and Cerium Oxide Nanoparticles as a Bioink for 3D Bioprinting.ACS applied nano materials · 2024Review
- 3D Bioprinting of Microbial-based Living Materials for Advanced Energy and Environmental Applications.Chem & bio engineering · 2024Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional (3D) bioprinting systems serve as advanced manufacturing platform for the precise deposition of cells and biomaterials at pre-defined positions. Among the various bioprinting techniques, the drop-on-demand jetting approach facilitates deposition of pico/nanoliter droplets of cells and materials for study of cell-cell and cell-matrix interactions. Despite advances in the bioprinting systems, there is a poor understanding of how the viability of primary human cells within sub-nanoliter droplets is affected during the printing process. In this work, a thermal inkjet system is utilized to dispense sub-nanoliter cell-laden droplets, and two key factors - droplet impact velocity and droplet volume - are identified to have significant effect on the viability and proliferation of printed cells. An increase in the cell concentration results in slower impact velocity, which leads to higher viability of the printed cells and improves the printing outcome by mitigating droplet splashing. Furthermore, a minimum droplet volume of 20 nL per spot helps to mitigate evaporation-induced cell damage and maintain high viability of the printed cells within a printing duration of 2 min. Hence, controlling the droplet impact velocity and droplet volume in sub-nanoliter bioprinting is critical for viability and proliferation of printed human primary cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.