ArticlePeerJ2022
Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies.
Article in PeerJ, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Acute nutritional ketosis during early recovery from aerobic exercise does not affect skeletal muscle transcriptomic response in humans.European journal of applied physiology · 2026Trial
- Sprint interval exercise disrupts mitochondrial ultrastructure driving a unique mitochondrial stress response and remodelling in men.Nature communications · 2025Trial
- Determinants of increased muscle insulin sensitivity of exercise-trained versus sedentary normal weight and overweight individuals.Science advances · 2025Trial
- Mechanical loading of bioengineered skeletal muscle in vitro recapitulates gene expression signatures of resistance exercise in vivo.Journal of cellular physiology · 2021Trial
- Omega-3 Supplementation Improves Isometric Strength But Not Muscle Anabolic and Catabolic Signaling in Response to Resistance Exercise in Healthy Older Adults.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Trial
- Conserved skeletal muscle transcriptomic responses to pacing strategies in Thoroughbred horses.Biology open · 2026Article
- Transcriptomic Profiling of the Biceps Femoris Long Head 72 h Following a Football Match and Associations With Recovery Biomarkers.Scandinavian journal of medicine & science in sports · 2026Article
- Cohort profile: The DIabetes and ST-segment Elevation Myocardial Infarction (DISTEMI) Study.Cardiovascular diabetology · 2026Observational
- Blood Biochemical Responses to Acute Exercise: Findings from the Molecular Transducers of Physical Activity Consortium (MoTrPAC).bioRxiv : the preprint server for biology · 2026Article
- Unilateral electrical stimulation of mice induces transcriptional response in stimulated leg with limited effect on non-stimulated contralateral leg.Experimental physiology · 2025Article
- Physical exercise to rebalance kynurenine metabolism in borderline personality disorder - Preliminary findings.Brain, behavior, & immunity - health · 2025Article
- Transcriptomic time course of skeletal muscle disuse and rehabilitation in middle-aged adults.Physiological reports · 2025Article
- Article
- Muscle Plasticity, Adaptation and Epigenetics.Advances in experimental medicine and biology · 2025Review
- The persistence of changes in insulin sensitivity and metabolic gene expression after acute exercise in male and female mice.Current research in physiology · 2025Article
- The 24-hour molecular landscape after exercise in humans reveals MYC is sufficient for muscle growth.EMBO reports · 2024Article
- Molecular aspects of the exercise response and training adaptation in skeletal muscle.Free radical biology & medicine · 2024Review
- Article
- Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal muscle.Pflugers Archiv : European journal of physiology · 2024Article
- The 24-Hour Time Course of Integrated Molecular Responses to Resistance Exercise in Human Skeletal Muscle ImplicatesbioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExercise elicits a range of adaptive responses in skeletal muscle, which include changes in mRNA expression. To better understand the health benefits of exercise training, it is important to investigate the underlying molecular mechanisms of skeletal muscle adaptation to exercise. However, most studies have assessed the molecular events at only a few time-points within a short time frame post-exercise, and the variations of gene expression kinetics have not been addressed systematically.
methodsWe assessed the mRNA expression of 23 gene isoforms implicated in the adaptive response to exercise at six time-points (0, 3, 9, 24, 48, and 72 h post exercise) over a 3-day period following a single session of high-intensity interval exercise.
resultsThe temporal patterns of target gene expression were highly variable and the expression of mRNA transcripts detected was largely dependent on the timing of muscle sampling. The largest fold change in mRNA expression of each tested target gene was observed between 3 and 72 h post-exercise. DISCUSSION AND
conclusionsOur findings highlight an important gap in knowledge regarding the molecular response to exercise, where the use of limited time-points within a short period post-exercise has led to an incomplete understanding of the molecular response to exercise. Muscle sampling timing for individual studies needs to be carefully chosen based on existing literature and preliminary analysis of the molecular targets of interest. We propose that a comprehensive time-course analysis on the exercise-induced transcriptional response in humans will significantly benefit the field of exercise molecular biology.
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