ArticleJournal of applied microbiology2022
Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridioides) difficile from the environment in Western Australia.
Article in Journal of applied microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Colonization Dynamics ofVeterinary sciences · 2026Article
- Clinical and Molecular Advances on the Black YeastInternational journal of molecular sciences · 2025Review
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- Distribution ofEmerging microbes & infections · 2024Article
- A Severe Infection Caused by a White Colony-Producing Strain ofAnnals of laboratory medicine · 2024Article
- The Brief Case: a White-Colony-Producing Clostridioides difficile Ribotype 020 Strain.Journal of clinical microbiology · 2023Review
- Complete Genome Sequences of Evolutionary Clade C-III Strains ofMicrobiology resource announcements · 2023Article
- Esculin hydrolysis negative and TcdA-only producing strains of Clostridium (Clostridioides) difficile from the environment in Western Australia.Journal of applied microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
BACKGROUND AND
aimsClostridium (Clostridiodes) difficile clade 3 ribotype (RT) 023 strains that fail to produce black colonies on bioMérieux ChromID agar have been reported, as well as variant strains of C. difficile that produce only toxin A. We have recently isolated strains of C. difficile from the environment in Western Australia (WA) with similar characteristics. The objective of this study was to characterize these strains. It was hypothesized that a putative β-glucosidase gene was lacking in these strains of C. difficile, including RT 023, leading to white colonies. METHODS AND
resultsA total of 17 environmental isolates of C. difficile from garden soil and compost, and gardening shoe soles in Perth, WA, failed to produce black colonies on ChromID agar. MALDI-TOF MS analysis confirmed these strains as C. difficile. Four strains contained only a tcdA gene (A
conclusionsChromID C. difficile agar is used for the specific detection of C. difficile in the samples. To date, all strains except RT 023 strains from clinical samples hydrolyse esculin. This is the first report to provide insights into the identification of esculin hydrolysis negative and TcdA-only producing (A SIGNIFICANCE AND IMPACT OF THE STUDY: White colonies of C. difficile from environmental samples could be overlooked when using ChromID C. difficile agar, leading to false-negative results, however, whether these strains are truly pathogenic remains to be proven.
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