Evidence map›Paper›PMID 35184249›Full record

ArticleFish physiology and biochemistry2022

Cloning and expression of kiss genes and regulation of feeding in Siberian sturgeon (Acipenser baerii).

Shaoqi Xu, Mei Wang, Ya Li, Ni Tang, Xin Zhang, Hu Chen, Shupeng Zhang, Yanling Liu, Jun Wang, Defang Chen and 1 more

Abstract read
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In one paragraph

Article in Fish physiology and biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Shaoqi XuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Mei WangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Ya LiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Ni TangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Xin ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Hu ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Shupeng ZhangCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Yanling LiuCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Jun WangKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, Sichuan, People's Republic of China.
Defang ChenCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Zhiqiong LiCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China. lizhiqiong454@163.com.
Sichuan Agricultural University · CNNeijiang Normal University · CN

Funding

Department of Science and Technology of Sichuan Province 2019YJ0438
6 · The paper itself

Abstract

In 1996, kiss was reported to regulate feeding in mammals, but studies are limited in fish. Our study aimed to explore the possible role of kiss in the regulation of feeding in Siberian sturgeon (Acipenser baerii). kiss1 and kiss2 were cloned, and the expression patterns were analyzed in Siberian sturgeon. The complete coding regions of kiss1 and kiss2 genes were 393 and 471 bp. Both kiss1 and kiss2 showed the highest expression level in the hypothalamus. During the periprandial and fasting experiments, the expression of kiss1 and kiss2 highly significantly increased in the hypothalamus after feeding (P < 0.01). Compared with the feeding group, in hypothalamus, kiss1 expression in the fasting group highly significantly decreased (P < 0.01). In contrast, kiss2 expression had no significant difference on days 1 and 7 (P > 0.05) but highly significantly increased on day 14 (P < 0.01). Subsequently, the feeding function was verified by intraperitoneal (i.p.) injection of Kp1(10) and Kp1(10) into fish. The results showed that i.p. injection of 1 µg/g BW Kp1(10) or 0.01 µg/g BW Kp2(10) could significantly reduce 0-1 h food intake (P < 0.05) and affected the expression levels of apelin, ghrelin, leptin, nmu, etc. in the hypothalamus. These results suggested that kiss1 plays an anorexic role in both short- and long-term feeding regulation, while kiss2 plays a short-term anorexic and long-term orexigenic role. This study described kiss as a novel regulator of appetite in fish and laid the groundwork for further studies focused on physiological function. HIGHLIGHTS: • The kiss1 and kiss2 of Siberian sturgeon were cloned. • The expression levels of kiss1 and kiss2 mRNA were the highest in the hypothalamus. • Postprandial hypothalamic kiss1 and kiss2 expression levels increased in the periprandial experiment. • In the fasting test, the expression of hypothalamic kiss1 decreased after fasting, while the expression of kiss2 increased after fasting on the 14th day. • Siberian sturgeon food intake was reduced, and appetite factors expression levels in the hypothalamus were altered after intraperitoneal injection of Kp1(10) and Kp2(10).

Indexed as

FishesKisspeptinsAnimalsAppetiteCloning, MolecularMammalsRNA, MessengerKisspeptinsRNA, MessengerAppetiteFood intakeKissSiberian sturgeon

Identifiers

PMID35184249
OpenAlexW4212837853

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.