Evidence map›Paper›PMID 35183243›Full record

ArticleJournal of ovarian research2022

Detection of plasma exosomal miRNA-205 as a biomarker for early diagnosis and an adjuvant indicator of ovarian cancer staging.

Zehua Zhu, Zhaojun Chen, Mingxing Wang, Min Zhang, Yiwen Chen, Xiao Yang, Changjun Zhou, Yuhua Liu, Liquan Hong, Lahong Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed, 3 pooled it
4.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 3 syntheses or guidelines pooled it, 59 citations in OpenAlex.

  1. Pooled it
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  10. The RNA content of extracellular vesicles from gene-editedMolecular therapy. Methods & clinical development · 2025
    Article
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  12. Exosomal ncRNAs in reproductive cancers†.Biology of reproduction · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Zehua ZhuThe Medical School of Hangzhou Normal University, Hangzhou, 310015, China.
Zhaojun ChenThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China.
Mingxing WangThe Medical School of Hangzhou Normal University, Hangzhou, 310015, China.
Min ZhangInstitute of Basic Medicine and Cancer of The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, 310022, China.
Yiwen ChenThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China.
Xiao YangThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China.
Changjun ZhouThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China.
Yuhua LiuThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China.
Liquan HongThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China.
Lahong ZhangThe Clinical laboratory of The Affiliated Hospital of Hangzhou Normal University, Hangzhou, 310015, China. zjhzzlh2007@163.com.ORCID http://orcid.org/0000-0002-6629-1045
Affiliated Hospital of Hangzhou Normal University · CNHangzhou Normal University · CNZhejiang Cancer Hospital · CN

Funding

Hangzhou Science and Technology Plan Development Project Fund 20191203B106Hangzhou Science and Technology Plan Development Project Fund 20201203B206Zhejiang Medical and Health Science and Technology Program Fund 2019KY131Zhejiang Medical and Health Science and Technology Program Fund 2019KY133
6 · The paper itself

Abstract

backgroundOvarian cancer (OC) is one of the serious threats to the health of women worldwide, and accurate biomarkers are urgently demanded for early diagnosis of OC. We have previously confirmed that miR-205 promotes the invasion and metastasis of OC cells by inhibiting the expression of the tumor suppressor gene TCF21. In this study, we used liquid biopsy technology to detect the expression levels of the four genes, miR-205, CA125, HE4 and TCF21, in the exosomes of plasma of OC patients. Combined with analysis of clinicopathological parameters of OC patients, we aimed to provide efficient and non-invasive laboratory biomarkers for early diagnosis of OC.

methods36 OC patients who were diagnosed in local hospitals from September 2020 to July 2021 were selected as OC group, 31 cases of surgically diagnosed with ovarian benign lesions were selected as benign group, and 32 healthy people who underwent physical examination during the same period were selected as a control group. We employed transmission electron microscope (TEM), Western blotting (WB), and nanoparticle tracking analysis (NTA) to identify biomarkers in the exosomes extracted from plasma of the three groups. The RNA levels of miR-205, CA125, HE4 and TCF21 genes in plasma exosomes were detected by real-time quantitative PCR (qRT-PCR) method. We used clinical pathological parameters and the Receiver Operating Characteristic (ROC) curves to evaluate the diagnostic efficacy for the genes detected in plasma exosomes.

resultsWe found that the expression level of miR-205 in plasma exosomes of the OC group was significantly higher than that of the benign and control groups (P <  0.05), and the level of miR-205 was elevated during the III-IV periods of OC and lymph node metastasis.

conclusionThe level of miR-205 in plasma exosomes is a valuable tumor biomarker to improve OC diagnosis.

Indexed as

AdultAgedAged, 80 and overArea Under CurveBasic Helix-Loop-Helix ProteinsCA-125 AntigenCase-Control StudiesEarly Detection of CancerExosomesFemaleHumansLiquid BiopsyLymphatic MetastasisMembrane ProteinsMicroRNAsMiddle AgedBasic Helix-Loop-Helix ProteinsCA-125 AntigenMembrane ProteinsMicroRNAsMIR205, humanMUC16 protein, humanTCF21 protein, humanWAP Four-Disulfide Core Domain Protein 2WFDC2 protein, humanBiomarkerDiagnosisExosomemiRNA-205Ovarian cancer

Identifiers

PMID35183243
PMCPMC8858566
OpenAlexW4213242228

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.