ArticleMicrobial cell factories2022
Altering the fatty acid profile of Yarrowia lipolytica to mimic cocoa butter by genetic engineering of desaturases.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 43 citations in OpenAlex.
- Sustainable industrial crop-based strategies for cocoa butter substitutes: advances in plant, microbial, and lipid engineering.Food chemistry: X · 2026Review
- Cystofilobasidium ferigula co-production of lipids and carotenoids using crude glycerol.NPJ science of food · 2026Article
- Fatty acid synthesis: A critical factor determining mycelial growth rate inFood chemistry. Molecular sciences · 2025Article
- Influence of process parameters on single-cell oil production by Cutaneotrichosporon oleaginosus using response surface methodology.Biotechnology for biofuels and bioproducts · 2025Article
- Sustainable Lipid Production withMicroorganisms · 2025Review
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Tuning Fatty Acid Profile and Yield inBioengineering (Basel, Switzerland) · 2023Article
- Opposite effects of low-carbohydrate high-fat diet on metabolism in humans and mice.Lipids in health and disease · 2023Article
- Lipid Production from Native Oleaginous Yeasts Isolated from Southern Chilean Soil Cultivated in Industrial Vinasse Residues.Microorganisms · 2023Article
- Article
- EngineeringSynthetic and systems biotechnology · 2022Review
- Tailoring and optimizing fatty acid production by oleaginous yeasts through the systematic exploration of their physiological fitness.Microbial cell factories · 2022Article
- Lipid production from lignocellulosic biomass using an engineered Yarrowia lipolytica strain.Microbial cell factories · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDemand for Cocoa butter is steadily increasing, but the supply of cocoa beans is naturally limited and under threat from global warming. One route to meeting the future demand for cocoa butter equivalent (CBE) could be to utilize microbial cell factories such as the oleaginous yeast Yarrowia lipolytica.
resultsThe main goal was to achieve triacyl-glycerol (TAG) storage lipids in Y. lipolytica mimicking cocoa butter. This was accomplished by replacing the native Δ9 fatty acid desaturase (Ole1p) with homologs from other species and changing the expression of both Ole1p and the Δ12 fatty acid desaturase (Fad2p). We thereby abolished the palmitoleic acid and reduced the linoleic acid content in TAG, while the oleic acid content was reduced to approximately 40 percent of the total fatty acids. The proportion of fatty acids in TAG changed dramatically over time during growth, and the fatty acid composition of TAG, free fatty acids and phospholipids was found to be very different.
conclusionsWe show that the fatty acid profile in the TAG of Y. lipolytica can be altered to mimic cocoa butter. We also demonstrate that a wide range of fatty acid profiles can be achieved while maintaining good growth and high lipid accumulation, which, together with the ability of Y. lipolytica to utilize a wide variety of carbon sources, opens up the path toward sustainable production of CBE and other food oils.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.