ArticleMicrobial cell factories2022
Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic media.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 58 citations in OpenAlex.
- CRISPR/Cas9 Genome Engineering in Non-Conventional Oleaginous Yeasts: Applications, Challenges, and Prospects.Yeast (Chichester, England) · 2026Review
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- Establishment of a protoplast transformation method for the oleaginous yeast Rhodotorula toruloides.FEMS yeast research · 2026Article
- Microbial synthesis and extraction of value-added metabolites by Rhodotorula toruloides from waste stream: a sustainable approach.Microbial cell factories · 2025Review
- Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder.Nature communications · 2025Article
- Recent advances in genetic engineering and chemical production in yeast species.FEMS yeast research · 2025Review
- Engineering of xylose metabolic pathways in Rhodotorula toruloides for sustainable biomanufacturing.FEMS yeast research · 2025Review
- Nitrogen starvation causes lipid remodeling in Rhodotorula toruloides.Microbial cell factories · 2024Article
- Enzymatic reactions towards aldehydes: An overview.Flavour and fragrance journal · 2023Review
- Lipid extract derived from newly isolatedComputational and structural biotechnology journal · 2023Article
- Spatial-temporal regulation of fatty alcohol biosynthesis in yeast.Biotechnology for biofuels and bioproducts · 2022Article
- Metabolic engineering of oleaginous yeast Rhodotorula toruloides for overproduction of triacetic acid lactone.Biotechnology and bioengineering · 2022Article
- Genetic manipulation of the interconversion between diacylglycerols and triacylglycerols inFrontiers in bioengineering and biotechnology · 2022Article
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7 authors at 2 institutions in 1 country.
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Abstract
backgroundThe oleaginous, carotenogenic yeast Rhodotorula toruloides has been increasingly explored as a platform organism for the production of terpenoids and fatty acid derivatives. Fatty alcohols, a fatty acid derivative widely used in the production of detergents and surfactants, can be produced microbially with the expression of a heterologous fatty acyl-CoA reductase. Due to its high lipid production, R. toruloides has high potential for fatty alcohol production, and in this study several metabolic engineering approaches were investigated to improve the titer of this product.
resultsFatty acyl-CoA reductase from Marinobacter aqueolei was co-expressed with SpCas9 in R. toruloides IFO0880 and a panel of gene overexpressions and Cas9-mediated gene deletions were explored to increase the fatty alcohol production. Two overexpression targets (ACL1 and ACC1, improving cytosolic acetyl-CoA and malonyl-CoA production, respectively) and two deletion targets (the acyltransferases DGA1 and LRO1) resulted in significant (1.8 to 4.4-fold) increases to the fatty alcohol titer in culture tubes. Combinatorial exploration of these modifications in bioreactor fermentation culminated in a 3.7 g/L fatty alcohol titer in the LRO1Δ mutant. As LRO1 deletion was not found to be beneficial for fatty alcohol production in other yeasts, a lipidomic comparison of the DGA1 and LRO1 knockout mutants was performed, finding that DGA1 is the primary acyltransferase responsible for triacylglyceride production in R. toruloides, while LRO1 disruption simultaneously improved fatty alcohol production, increased diacylglyceride and triacylglyceride production, and increased glucose consumption.
conclusionsThe fatty alcohol titer of fatty acyl-CoA reductase-expressing R. toruloides was significantly improved through the deletion of LRO1, or the deletion of DGA1 combined with overexpression of ACC1 and ACL1. Disruption of LRO1 surprisingly increased both lipid and fatty alcohol production, creating a possible avenue for future study of the lipid metabolism of this yeast.
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