ArticleAdvanced materials (Deerfield Beach, Fla.)2022
In Situ Super-Resolution Imaging of Organoids and Extracellular Matrix Interactions via Phototransfer by Allyl Sulfide Exchange-Expansion Microscopy (PhASE-ExM).
Article in Advanced materials (Deerfield Beach, Fla.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Photoresponsive Granular Hydrogels Enable Spatiotemporal Control of Matrix Mechanics and MSC Behavior.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- HiExM Enables Scalable Mapping of Organelle Morphology and Spatial Heterogeneity.bioRxiv : the preprint server for biology · 2026Article
- Controlling Intestinal Organoid Polarity using Synthetic Dynamic Hydrogels Decorated with Laminin-Derived IKVAV Peptides.Advanced healthcare materials · 2026Article
- Advanced Microscopy Techniques.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer · 2026Review
- From Light to Life: Molecular Mechanisms and Macroscopic Transformations in Photoresponsive Hydrogels.Polymer science & technology (Washington, D.C.) · 2025Review
- Nuclei sense complex tissue shape and direct intestinal stem cell fate.bioRxiv : the preprint server for biology · 2025Article
- Fully Synthetic Hydrogels Promote Robust Crypt Formation in Intestinal Organoids.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Formulation Strategies for Immunomodulatory Natural Products in 3D Tumor Spheroids and Organoids: Current Challenges and Emerging Solutions.Pharmaceutics · 2025Review
- Study on the Feasibility of Self-Assembling Peptides as a Three-Dimensional Culture Tool for Drug Screening of Colorectal Adenocarcinoma Cells.Gels (Basel, Switzerland) · 2025Article
- Engineered epithelial curvature controls Paneth cell localization in intestinal organoids.Cell biomaterials · 2025Article
- Bio-orthogonal Glycan Imaging of Cultured Cells and Whole AnimalACS central science · 2025Article
- Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels.Stem cell reports · 2025Article
- Photoresponsive Chemistries for User-Directed Hydrogel Network Modulation to Investigate Cell-Matrix Interactions.Accounts of chemical research · 2025Review
- Polymer design of microwell hydrogels influences epithelial-mesenchymal interactions during human bronchosphere formation.Advanced nanobiomed research · 2025Article
- Harnessing the power of artificial intelligence for human living organoid research.Bioactive materials · 2024Review
- Article
- HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging.bioRxiv : the preprint server for biology · 2024Article
- Bioengineering approaches for the endometrial research and application.Materials today. Bio · 2024Review
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications.Advanced drug delivery reviews · 2024Review
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Authors and funding
6 authors.
Funding
Abstract
3D organoid models have recently seen a boom in popularity, as they can better recapitulate the complexity of multicellular organs compared to other in vitro culture systems. However, organoids are difficult to image because of the limited penetration depth of high-resolution microscopes and depth-dependent light attenuation, which can limit the understanding of signal transduction pathways and characterization of intimate cell-extracellular matrix (ECM) interactions. To overcome these challenges, phototransfer by allyl sulfide exchange-expansion microscopy (PhASE-ExM) is developed, enabling optical clearance and super-resolution imaging of organoids and their ECM in 3D. PhASE-ExM uses hydrogels prepared via photoinitiated polymerization, which is advantageous as it decouples monomer diffusion into thick organoid cultures from the hydrogel fabrication. Apart from compatibility with organoids cultured in Matrigel, PhASE-ExM enables 3.25× expansion and super-resolution imaging of organoids cultured in synthetic poly(ethylene glycol) (PEG) hydrogels crosslinked via allyl-sulfide groups (PEG-AlS) through simultaneous photopolymerization and radical-mediated chain-transfer reactions that complete in <70 s. Further, PEG-AlS hydrogels can be in situ softened to promote organoid crypt formation, providing a super-resolution imaging platform both for pre- and post-differentiated organoids. Overall, PhASE-ExM is a useful tool to decipher organoid behavior by enabling sub-micrometer scale, 3D visualization of proteins and signal transduction pathways.
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