Evidence map›Paper›PMID 35181706›Full record

ArticleScientific reports2022

RNA-Seq analysis of a Pax3-expressing myoblast clone in-vitro and effect of culture surface stiffness on differentiation.

Louise Richardson, Dapeng Wang, Ruth Hughes, Colin A Johnson, Michelle Peckham

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Louise RichardsonSchool of Molecular and Cellular Biology, University of Leeds, Leeds, UK.
Dapeng WangLeedsOmics, University of Leeds, Leeds, LS2 9JT, UK.
Ruth HughesSchool of Molecular and Cellular Biology, University of Leeds, Leeds, UK.
Colin A JohnsonLeeds Institute of Medical Research, Faculty of Medicine and Health, University of Leeds, Leeds, UK.
Michelle PeckhamSchool of Molecular and Cellular Biology, University of Leeds, Leeds, UK. m.peckham@leeds.ac.uk.
University of Leeds · GBCentre for Human Genetics · GB

Funding

Biotechnology and Biological Sciences Research Council BB/M011151/1Biotechnology and Biological Sciences Research Council BB/P007791/1Medical Research Council 1233630Medical Research Council MR/M000532/1Wellcome TrustWellcome Trust WT104918MA
6 · The paper itself

Abstract

Skeletal muscle satellite cells cultured on soft surfaces (12 kPa) show improved differentiation than cells cultured on stiff surfaces (approximately 100 kPa). To better understand the reasons for this, we performed an RNA-Seq analysis for a single satellite cell clone (C1F) derived from the H2k

Indexed as

Cell Culture TechniquesSurface PropertiesAnimalsCell DifferentiationCell ProliferationGene Expression Regulation, DevelopmentalMiceMuscle Fibers, SkeletalMyoblastsPAX3 Transcription FactorRNA-SeqSatellite Cells, Skeletal MuscleSingle-Cell AnalysisPax3 protein, mousePAX3 Transcription Factor

Identifiers

PMID35181706
PMCPMC8857316
OpenAlexW4220851386

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.