ArticleProceedings of the National Academy of Sciences of the United States of America2022
The mouse brain after foot shock in four dimensions: Temporal dynamics at a single-cell resolution.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Reduced MOV10 reveals novel functional cortical connections in an increased fear response.bioRxiv : the preprint server for biology · 2026Article
- Mouse Bio-behavioral Phenotyping Using a Digital Homecage Framework for Long-timescale, High-resolution, and Multi-factor Data Collection and Analytics.Research square · 2026Article
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- Prelimbic cortex, but not its nucleus reuniens projections, regulates punished alcohol self-administration.Neuropharmacology · 2026Article
- Review
- Article
- Immediate-early genes Arc and c-Fos show divergent brain-wide expression following contextual fear conditioning.Communications biology · 2025Article
- Short-term stress preconditioning activates sympathetic innervation in the testes to mitigate testicular ischemia-reperfusion injury.Journal of neuroinflammation · 2025Article
- Dynamics of stress-inducedNeurobiology of stress · 2025Article
- Sex-specific fear acquisition following early life stress is linked to amygdala and hippocampal purine and glutamate metabolism.Communications biology · 2024Article
- Identification of a stress-responsive subregion of the basolateral amygdala in male rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders.Molecular psychiatry · 2024Review
- Evidence for a Role of 5-HT-glutamate Co-releasing Neurons in Acute Stress Mechanisms.ACS chemical neuroscience · 2024Article
- The cortisol switch between vulnerability and resilience.Molecular psychiatry · 2024Review
- Shared and Distinct Brain Regions Targeted for Immediate Early Gene Expression by Ketamine and Psilocybin.ACS chemical neuroscience · 2023Article
- Overnight Corticosterone and Gene Expression in Mouse Hippocampus: Time Course during Resting Period.International journal of molecular sciences · 2023Article
- Whole-brain tracking of cocaine and sugar rewards processing.Translational psychiatry · 2023Article
- Tissue clearing applications in memory engram research.Frontiers in behavioral neuroscience · 2023Review
- Brain mineralocorticoid and glucocorticoid receptor balance in neuroendocrine regulation and stress-related psychiatric etiopathologies.Current opinion in endocrine and metabolic research · 2022Review
- Mineralocorticoid receptor and glucocorticoid receptor work alone and together in cell-type-specific manner: Implications for resilience prediction and targeted therapy.Neurobiology of stress · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acute stress leads to sequential activation of functional brain networks. A biologically relevant question is exactly which (single) cells belonging to brain networks are changed in activity over time after acute stress across the entire brain. We developed a preprocessing and analytical pipeline to chart whole-brain immediate early genes' expression-as proxy for cellular activity-after a single stressful foot shock in four dimensions: that is, from functional networks up to three-dimensional (3D) single-cell resolution and over time. The pipeline is available as an R package. Most brain areas (96%) showed increased numbers of c-fos+ cells after foot shock, yet hypothalamic areas stood out as being most active and prompt in their activation, followed by amygdalar, prefrontal, hippocampal, and finally, thalamic areas. At the cellular level, c-fos+ density clearly shifted over time across subareas, as illustrated for the basolateral amygdala. Moreover, some brain areas showed increased numbers of c-fos+ cells, while others-like the dentate gyrus-dramatically increased c-fos intensity in just a subset of cells, reminiscent of engrams; importantly, this "strategy" changed after foot shock in half of the brain areas. One of the strengths of our approach is that single-cell data were simultaneously examined across all of the 90 brain areas and can be visualized in 3D in our interactive web portal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.