ArticleScience (New York, N.Y.)1986
Brain glutamate decarboxylase cloned in lambda gt-11: fusion protein produces gamma-aminobutyric acid.
Article in Science (New York, N.Y.), 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 262 citations in OpenAlex.
- Presynaptic Trafficking of Glutamate Decarboxylase Isoforms Is Dispensable for Basal GABAergic Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Cardiac endogenous transmitter system: molecular features, functions, and clinical implications.European heart journal · 2026Review
- Article
- Glutamatergic Projections from the Entorhinal Cortex to Dorsal Dentate Gyrus Mediate Context-Induced Reinstatement of Heroin Seeking.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2017Article
- Clinically applicable GABA receptor positive allosteric modulators promote ß-cell replication.Scientific reports · 2017Article
- Article
- Cell Type-Specific Structural Organization of the Six Layers in Rat Barrel Cortex.Frontiers in neuroanatomy · 2017Review
- Article
- Generation of dense statistical connectomes from sparse morphological data.Frontiers in neuroanatomy · 2014Article
- Cellular organization of cortical barrel columns is whisker-specific.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Role of orbitofrontal cortex neuronal ensembles in the expression of incubation of heroin craving.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Lack of molecular-anatomical evidence for GABAergic influence on axon initial segment of cerebellar Purkinje cells by the pinceau formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- GABA and GAD expression in the X-organ sinus gland system of the Procambarus clarkii crayfish: inhibition mediated by GABA between X-organ neurons.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2011Article
- Spatial distribution of neural activity in the anterior olfactory nucleus evoked by odor and electrical stimulation.The Journal of comparative neurology · 2011Article
- Number and laminar distribution of neurons in a thalamocortical projection column of rat vibrissal cortex.Cerebral cortex (New York, N.Y. : 1991) · 2010Article
- Heterogeneity of the supramammillary-hippocampal pathways: evidence for a unique GABAergic neurotransmitter phenotype and regional differences.The European journal of neuroscience · 2010Article
- NR2 subunits and NMDA receptors on lamina II inhibitory and excitatory interneurons of the mouse dorsal horn.Molecular pain · 2010Article
- Proopiomelanocortin expression in both GABA and glutamate neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Low-threshold primary afferent drive onto GABAergic interneurons in the superficial dorsal horn of the mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glutamate decarboxylase (GAD; E.C. 4.1.1.15) converts glutamate to gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the vertebrate central nervous system. This report describes the isolation of a GAD complementary DNA clone by immunological screening of a lambda gt-11 brain complementary DNA expression library. The fusion protein produced by this clone catalyzes the conversion of glutamate to GABA and carbon dioxide, confirming its identity as GAD. Antibodies to beta-galactosidase remove GAD enzymatic activity from solution, showing that this activity is associated with the fusion protein. In immunoblotting experiments all three available antisera to GAD reacted with the fusion polypeptide and with two major polypeptides (molecular size, 60,000 and 66,000 daltons) in brain extracts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.