ArticleStem cell reports2022
Long-term adherence of human brain cells in vitro is enhanced by charged amine-based plasma polymer coatings.
Article in Stem cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Drug screen and machine learning predict neuroprotective agents in a preclinical human model of childhood dementia.Nature communications · 2026Article
- Radical-Mediated, Substrate-Independent Fabrication of Hybrid Solid-Hydrogel Materials With Tunable Crosslinking: An Initiator- and Crosslinker-Free Approach.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Modelling synaptic dysfunction in childhood dementia using human iPSC-derived cortical networks.Nature communications · 2026Article
- A programmable peptide interface for on-demand neural culturing platforms.Journal of nanobiotechnology · 2026Article
- Optimizing the in vitro neuronal microenvironment to mitigate phototoxicity in live-cell imaging.Stem cell research & therapy · 2025Article
- Aberrant neuronal firing: a paracrine route to glioblastoma expansion.Cell communication and signaling : CCS · 2025Article
- Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection.ACS applied materials & interfaces · 2024Article
- Plasma-Activated Coated Glass: A Novel Platform for Optimal Optical Performance and Cell Culture Substrate Customization.Small science · 2024Article
- A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness.Biomimetics (Basel, Switzerland) · 2023Article
- Article
- Electroactive Materials Surface Charge Impacts Neuron Viability and Maturation in 2D Cultures.ACS applied materials & interfaces · 2023Article
- An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids.Cells · 2023Article
- Engineering circuits of human iPSC-derived neurons and rat primary glia.Frontiers in neuroscience · 2023Article
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Authors and funding
18 authors.
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Abstract
Advances in cellular reprogramming have radically increased the use of patient-derived cells for neurological research in vitro. However, adherence of human neurons on tissue cultureware is unreliable over the extended periods required for electrophysiological maturation. Adherence issues are particularly prominent for transferable glass coverslips, hindering imaging and electrophysiological assays. Here, we assessed thin-film plasma polymer treatments, polymeric factors, and extracellular matrix coatings for extending the adherence of human neuronal cultures on glass. We find that positive-charged, amine-based plasma polymers improve the adherence of a range of human brain cells. Diaminopropane (DAP) treatment with laminin-based coating optimally supports long-term maturation of fundamental ion channel properties and synaptic activity of human neurons. As proof of concept, we demonstrated that DAP-treated glass is ideal for live imaging, patch-clamping, and optogenetics. A DAP-treated glass surface reduces the technical variability of human neuronal models and enhances electrophysiological maturation, allowing more reliable discoveries of treatments for neurological and psychiatric disorders.
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