Evidence map›Paper›PMID 35179654›Full record

ArticleArchives of microbiology2022

Nanoencapsulated Lippia rotundifolia antimicrobial peptide: synthesis, characterization, antimicrobial activity, and cytotoxicity evaluations.

Elisandra M Dos Santos, Letícia S Tavares, Leonara Fayer, Humberto M Brandão, Michele Munk, Marcelo de O Santos

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Elisandra M Dos SantosLaboratory of g Genetics and Biotechnology, Federal University of Juiz de Fora, Juiz de Fora, MG, 36036-900, Brazil.
Letícia S TavaresLaboratory of g Genetics and Biotechnology, Federal University of Juiz de Fora, Juiz de Fora, MG, 36036-900, Brazil.
Leonara FayerLaboratory of g Genetics and Biotechnology, Federal University of Juiz de Fora, Juiz de Fora, MG, 36036-900, Brazil.
Humberto M BrandãoLaboratory of Nanotechnology, Brazilian Agricultural Research Corporation (EMBRAPA), Juiz de Fora, MG, 36038-330, Brazil.
Michele MunkLaboratory of g Genetics and Biotechnology, Federal University of Juiz de Fora, Juiz de Fora, MG, 36036-900, Brazil.
Marcelo de O SantosLaboratory of g Genetics and Biotechnology, Federal University of Juiz de Fora, Juiz de Fora, MG, 36036-900, Brazil. marcelo.santos@ufjf.edu.br.ORCID http://orcid.org/0000-0003-4909-2252
Universidade Federal de Juiz de Fora · BRBrazilian Agricultural Research Corporation · BR

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 405030/2015-0Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Financial code: 001Fundação de Amparo à Pesquisa do Estado de Minas Gerais RED-00282-16
6 · The paper itself

Abstract

Antimicrobial peptides (AMP) are promising novel antibiotics but exhibit low stability and can be toxic. The AMP encapsulation can be used to protect the drug and control its release rates. The Lr-AMP1f encapsulated into chitosan nanoparticle (NP) by ionic gelation method reached 90% efficiency. The results indicated that the hydrodynamic particle size of NPs increased from 196.1 ± 3.14 nm (free NP) to 228.1 ± 12.22 nm (nanoencapsulated Lr-AMP1f), while the atomic force microscope showed the spherical shape. The Zeta potential of the nanoencapsulated Lr-AMP1f was high (+ 35 mV). These AMP-loaded NPs exhibited stability for up to 21 days of storage. The minimum inhibitory concentration (MIC) of free Lr-AMP1f was 8 µg/mL for E. coli and S. epidermidis. However, the nanoencapsulated Lr-AMP1f produced a bacteriostatic effect against both bacteria at 8 µg/mL. The MIC of nanoencapsulated Lr-AMP1f was 16 µg/mL for E. coli and 32 for S. epidermidis. Nanoencapsulated Lr-AMP1f was nontoxic to HEK293 cells. Promisingly, chitosan NP can be used as a vehicle for the antibacterial application of new AMP (Lr-AMP1f).

Indexed as

ChitosanLippiaAnti-Bacterial AgentsAntimicrobial PeptidesEscherichia coliHEK293 CellsHumansParticle SizeAnti-Bacterial AgentsAntimicrobial PeptidesChitosanAntimicrobial peptidesNanocapsulatesNanotechnology

Identifiers

PMID35179654
OpenAlexW4212946488

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.