ArticlePlant biotechnology journal2022
All-in-one: a robust fluorescent fusion protein vector toolbox for protein localization and BiFC analyses in plants.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Transcriptional and Functional Insights Into the All-Stage Resistance Gene GmRmd1-Mediated Regulation of Resistance to Powdery Mildew in Soybean.Molecular plant pathology · 2026Article
- Multiplexed Engineering of Disease Resistance and Lodging Tolerance in Wheat Via a Single Xylan Modifying Glycosyltransferase.Plant biotechnology journal · 2026Article
- Alternatively spliced killer-protector system confers S19-mediated hybrid male sterility in rice.Nature communications · 2026Article
- Overexpression of OsAGO18 Promotes Early Seedling Development and Root Elongation in Rice.Plants (Basel, Switzerland) · 2026Article
- Sorghum bicolor membrane steroid binding protein 1 can bind heme and remodel ER membranes.Plant & cell physiology · 2026Article
- Alternaria alternata Effector Aa593 Promotes Virulence by Hijacking the CmNAC29-Mediated Abscisic Acid Biosynthesis Pathway in Chrysanthemum.Plant biotechnology journal · 2026Article
- Gene Editing of a Susceptibility LncRNA Enhances Broad-Spectrum Disease Resistance in Rice without Developmental Trade-Offs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Ubiquitin-mediated degradation restricts spatiotemporal accumulation of the cytoplasmic male sterility protein WA352 to anthers in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- pMAGs: A Versatile and Efficient Vector System for Multi-Gene Studies in Plants.Plants (Basel, Switzerland) · 2025Article
- An all-in-one plant virus-based vector toolkit for streamlined gene silencing, overexpression, and genome editing.Plant communications · 2025Article
- Spinach SoNRT3 Interacts with SoNRT2a to Improve Low-Nitrogen Tolerance via Nitrate Uptake and Root Growth.Plants (Basel, Switzerland) · 2025Article
- Overexpression of GFP Fusions of Regulators of the SAC from Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Haplotype-resolved genome assembly and resequencing analysis provide insights into genome evolution and allelic imbalance in Pinus densiflora.Nature genetics · 2024Article
- Comprehensive Review on Bimolecular Fluorescence Complementation and Its Application in Deciphering Protein-Protein Interactions in Cell Signaling Pathways.Biomolecules · 2024Review
- Identification of tomato F-box proteins functioning in phenylpropanoid metabolism.Plant molecular biology · 2024Article
- A Mitochondrial Localized Chaperone Regulator OsBAG6 Functions in Saline-Alkaline Stress Tolerance in Rice.Rice (New York, N.Y.) · 2024Article
- Targeted mRNA demethylation in Arabidopsis using plant m6A editor.Plant methods · 2023Article
- Two complementary genes in a presence-absence variation contribute to indica-japonica reproductive isolation in rice.Nature communications · 2023Article
- A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants.Plant communications · 2023Article
- Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation.Plant communications · 2022Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent tagging protein localization (FTPL) and bimolecular fluorescence complementation (BiFC) are popular tools for in vivo analyses of the subcellular localizations of proteins and protein-protein interactions in plant cells. The efficiency of fluorescent fusion protein (FFP) expression analyses is typically impaired when the FFP genes are co-transformed on separate plasmids compared to when all are cloned and transformed in a single vector. Functional genomics applications using FFPs such as a gene family studies also often require the generation of multiple plasmids. Here, to address these needs, we developed an efficient, modular all-in-one (Aio) FFP (AioFFP) vector toolbox, including a set of fluorescently labelled organelle markers, FTPL and BiFC plasmids and associated binary vectors. This toolbox uses Gibson assembly (GA) and incorporates multiple unique nucleotide sequences (UNSs) to facilitate efficient gene cloning. In brief, this system enables convenient cloning of a target gene into various FFP vectors or the insertion of two or more target genes into the same FFP vector in a single-tube GA reaction. This system also enables integration of organelle marker genes or fluorescently fused target gene expression units into a single transient expression plasmid or binary vector. We validated the AioFFP system by testing genes encoding proteins known to be functional in FTPL and BiFC assays. In addition, we performed a high-throughput assessment of the accurate subcellular localizations of an uncharacterized rice CBSX protein subfamily. This modular UNS-guided GA-mediated AioFFP vector toolkit is cost-effective, easy to use and will promote functional genomics research in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.