ArticleFrontiers in cellular and infection microbiology2022
A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic
Article in Frontiers in cellular and infection microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Limitations of Multiplex Polymerase Chain Reaction in Urinary Tract Infection Diagnosis and the Need for Inclusive Research.International urogynecology journal · 2026Article
- Methodological bias shapes the interpretation of the urinary microbiome in low-biomass systems.Frontiers in microbiology · 2026Review
- Application-focused validation of a viability-selective PMA-qPCR assay for selected skin-associated and cosmetic-relevant bacteria.Frontiers in microbiology · 2026Article
- Improving biocide evaluation using propidium monoazide (PMA) viability staining technique.Scientific reports · 2025Article
- Rewriting the urinary tract paradigm: the urobiome as a gatekeeper of host defense.Molecular biology reports · 2025Review
- Metabolic characterization of alkane monooxygenases and the growth phenotypes ofJournal of bacteriology · 2025Article
- Propidium Monoazide is Unreliable for Quantitative Live-Dead Molecular Assays.Analytical chemistry · 2025Article
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR.Biosafety and health · 2024Article
- Improving the Efficiency of Viability-qPCR with Lactic Acid Enhancer for the Selective Detection of Live Pathogens in Foods.Foods (Basel, Switzerland) · 2024Article
- A Pilot Study to Detect ViableInternational journal of molecular sciences · 2023Article
- Rapid Molecular Phenotypic Antimicrobial Susceptibility Test forACS infectious diseases · 2023Article
- Rapid molecular phenotypic antimicrobial susceptibility test forbioRxiv : the preprint server for biology · 2023Article
- Recent Methods for the Viability Assessment of Bacterial Pathogens: Advances, Challenges, and Future Perspectives.Pathogens (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPolymerase chain reaction (PCR) is an important means by which to study the urine microbiome and is emerging as possible alternative to urine cultures to identify pathogens that cause urinary tract infection (UTI). However, PCR is limited by its inability to differentiate DNA originating from viable, metabolically active versus non-viable, inactive bacteria. This drawback has led to concerns that urobiome studies and PCR-based diagnosis of UTI are confounded by the presence of relic DNA from non-viable bacteria in urine. Propidium monoazide (PMA) dye can penetrate cells with compromised cell membranes and covalently bind to DNA, rendering it inaccessible to amplification by PCR. Although PMA has been shown to differentiate between non-viable and viable bacteria in various settings, its effectiveness in urine has not been previously studied. We sought to investigate the ability of PMA to differentiate between viable and non-viable bacteria in urine.
methodsVarying amounts of viable or non-viable uropathogenic
resultsPMA's efficiency in excluding DNA signal from non-viable bacteria was significantly higher in bacterial samples in phosphate-buffered saline (PBS, dC
conclusionWe have successfully developed PMA-based PCR methods for amplifying DNA from live bacteria in urine. Our results suggest that non-PMA bound DNA from live bacteria can be present in urine, even after antibiotic treatment. This indicates that viable but non-culturable
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