Evidence map›Paper›PMID 35178295›Full record

ArticlePeerJ2022

In leukemia, knock-down of the death inducer-obliterator gene would inhibit the proliferation of endothelial cells by inhibiting the expression of

Honghua Cao, Lilan Wang, Chengkui Geng, Man Yang, Wenwen Mao, Linlin Yang, Yin Ma, Ming He, Yeying Zhou, Lianqing Liu and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Honghua Cao *Department of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Lilan Wang *Department of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Chengkui GengDepartment of Orthopedics, Yan'an Hospital of Kunming City, The Affiliated Hospital of Kunming Medical University, Kunming, China.
Man YangDepartment of Endocrinology, The Affiliated Hospital of Yunnan University & The Second People's Hospital of Yunnan Province, Kunming Yunnan, Kunming, Yunnan, China.
Wenwen MaoDepartment of Geriatics, The Second Hospital of Kunming, Kunming, China.
Linlin YangDepartment of Gynecology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Yin MaDepartment of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Ming HeDepartment of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Yeying ZhouDepartment of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Lianqing LiuDepartment of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Xuejiao HuDepartment of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Jingxing YuDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.ORCID 0000-0002-5157-1757
Xiufen ShenDepartment of Laboratory, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Xuezhong GuDepartment of Hematology, The First People Hospital in Yunnan Province, Kunming, China.
Liefen YinDepartment of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Zhenglei ShenDepartment of Hematology, The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Kunming Medical University · CNFirst People's Hospital of Yunnan Province · CNYunnan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEndothelial cells (ECs) are a critical component of the hematopoietic niche, and the cross-talk between ECs and leukemia was reported recently. This study aimed to determine the genes involved in the proliferation inhibition of endothelial cells in leukemia.

methodsHuman umbilical vein endothelial cells (HUVEC) were cultured alone or co-cultured with K562 cell lines. GeneChip assays were performed to identify the differentially expressed genes. The Celigo, MTT assay, and flow cytometric analysis were used to determine the effect of RNAi DIDO on cell growth and apoptosis. The differently expressed genes were verified by qRT-PCR (quantitative real-time PCR) and western-blot.

resultsIn K562-HUVEC co-cultured cell lines, 323 down-regulated probes were identified and the extracellular signal-regulated kinase 5 (ERK5) signaling pathway was significantly inhibited. Among the down-regulated genes, the

conclusionThe knock-down of

Indexed as

Cyclin D1LeukemiaCell ProliferationCyclin-Dependent Kinase 6HumansHuman Umbilical Vein Endothelial CellsCCND1 protein, humanCDK6 protein, humanCyclin D1Cyclin-Dependent Kinase 6CCND1CDK6DIDOEndothelial cellsLeukemia

Identifiers

PMID35178295
PMCPMC8815367
OpenAlexW4210425228

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.