ArticleCommunications biology2022
TAQing2.0 for genome reorganization of asexual industrial yeasts by direct protein transfection.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Genetic and phenotypic insights into Cyberlindnera jadinii as a promising yeast for industrial biotechnology.G3 (Bethesda, Md.) · 2025Article
- Light-controlled Spo11-less meiotic DNA breaks by MagTAQing lead to chromosomal aberrations.Nucleic acids research · 2025Article
- Advantages of Mutant Generation by Genome Rearrangements of Non-Conventional Yeast via Direct Nuclease Transfection.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Quantitative physiology and biomass composition of Cyberlindnera jadinii in ethanol-grown cultures.Biotechnology for biofuels and bioproducts · 2024Article
- Gene mapping methodology powered by induced genome rearrangements.Scientific reports · 2022Article
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Authors and funding
9 authors at 2 institutions in 1 country.
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Abstract
Genomic rearrangements often generate phenotypic diversification. We previously reported the TAQing system where genomic rearrangements are induced via conditional activation of a restriction endonuclease in yeast and plant cells to produce mutants with marked phenotypic changes. Here we developed the TAQing2.0 system based on the direct delivery of endonucleases into the cell nucleus by cell-penetrating peptides. Using the optimized procedure, we introduce a heat-reactivatable endonuclease TaqI into an asexual industrial yeast (torula yeast), followed by a transient heat activation of TaqI. TAQing2.0 leads to generation of mutants with altered flocculation and morphological phenotypes, which exhibit changes in chromosomal size. Genome resequencing suggested that torula yeast is triploid with six chromosomes and the mutants have multiple rearrangements including translocations having the TaqI recognition sequence at the break points. Thus, TAQing2.0 is expected as a useful method to obtain various mutants with altered phenotypes without introducing foreign DNA into asexual industrial microorganisms.
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