Evidence map›Paper›PMID 35177783›Full record

ArticleScientific reports2022

FTIR differentiation based on genomic DNA for species identification of Shigella isolates from stool samples.

Babak Pakbin, Leila Zolghadr, Shahnaz Rafiei, Wolfram Manuel Brück, Thomas B Brück

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.2field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. AnalyzingInfection and drug resistance · 2025
    Article
  4. Review
  5. Article
  6. Article
  7. Interactions ofMembranes · 2023
    Article
  8. Interaction of Positively Charged Oligopeptides with Blood Plasma Proteins.International journal of molecular sciences · 2023
    Article
  9. Molecular Mechanisms ofInternational journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Babak PakbinMedical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Leila ZolghadrChemistry Department, Imam Khomeini International University, Qazvin, Iran.
Shahnaz RafieiChemistry Department, Imam Khomeini International University, Qazvin, Iran.
Wolfram Manuel BrückInstitute for Life Technologies, University of Applied Sciences Western Switzerland Valais-Wallis, 1950, Sion 2, Switzerland. wolfram.bruck@hevs.ch.
Thomas B BrückWerner Siemens Chair of Synthetic Biotechnology, Department of Chemistry, Technical University of Munich (TUM), Lichtenberg Str. 4, 85748, Garching bei München, Germany.
HES-SO Valais-Wallis · CHImam Khomeini International University · IRSiemens (Germany) · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Shigellosis is one of the major public health concerns in developing and low-income countries caused by four species of Shigella. There is an apparent need to develop rapid, cost-effective, sensitive and specific methods for differentiation of Shigella species to be used in outbreaks and health surveillance systems. We developed a sensitive and specific Fourier-transform infrared spectroscopy (FTIR) based method followed by principal component analysis (PCA) and hierarchical clustering analysis (HCA) assays to differentiate four species of Shigella isolates from stool samples. The FTIR based method was evaluated by differentiation of 91 Shigella species from each other in clinical samples using both gold standards (culture-based and agglutination methods) and developed FTIR assay; eventually, the sensitivity and specificity of the developed method were calculated. In summary, four distinct FTIR spectra associated with four species of Shigella were obtained with wide variations in three definite regions, including 1800-1550 cm

Indexed as

DNA, BacterialShigellaAdultAgedChildFecesFemaleHumansMaleMiddle AgedSpectroscopy, Fourier Transform InfraredYoung AdultDNA, Bacterial

Identifiers

PMID35177783
PMCPMC8854563
OpenAlexW4221048666

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.