ArticleNPJ biofilms and microbiomes2022
Subsurface hydrocarbon degradation strategies in low- and high-sulfate coal seam communities identified with activity-based metagenomics.
Article in NPJ biofilms and microbiomes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Review
- Tracking active heterotrophic microbial communities in the Guaymas Basin deep biosphere with BONCAT-FACS.ISME communications · 2026Article
- Recent progress on the biological degradation and solubilization of coal.Biodegradation · 2025Review
- Functional group characteristics of coal treated with clean biomass surfactant via FTIR spectroscopy.Scientific reports · 2025Article
- Anthropogenic impacts on the terrestrial subsurface biosphere.Nature reviews. Microbiology · 2025Review
- Biotechnological potentials of surfactants in coal utilization: a review.Environmental science and pollution research international · 2024Review
- The hydrocarbon pollution crisis: Harnessing the earth hydrocarbon-degrading microbiome.Microbial biotechnology · 2024Article
- New insights into the coal-associated methane architect: the ancient archaebacteria.Archives of microbiology · 2024Review
- Article
- Selective trace elements significantly enhanced methane production in coal bed methane systems by stimulating microbial activity.Microbiology spectrum · 2024Article
- The Isolation of Anaerobic and Facultative Anaerobic Sulfate-Reducing Bacteria (SRB) and a Comparison of Related Enzymes in Their Sulfate Reduction Pathways.Microorganisms · 2023Article
- Metagenome analyses of microbial population in geotextile fabrics used in permeable reactor barriers for toluene biodegradation.3 Biotech · 2023Article
- Natural and anthropogenic carbon input affect microbial activity in salt marsh sediment.Frontiers in microbiology · 2023Article
- Algal amendment enhances biogenic methane production from coals of different thermal maturity.Frontiers in microbiology · 2023Article
- Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane.The ISME journal · 2022Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
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Abstract
Environmentally relevant metagenomes and BONCAT-FACS derived translationally active metagenomes from Powder River Basin coal seams were investigated to elucidate potential genes and functional groups involved in hydrocarbon degradation to methane in coal seams with high- and low-sulfate levels. An advanced subsurface environmental sampler allowed the establishment of coal-associated microbial communities under in situ conditions for metagenomic analyses from environmental and translationally active populations. Metagenomic sequencing demonstrated that biosurfactants, aerobic dioxygenases, and anaerobic phenol degradation pathways were present in active populations across the sampled coal seams. In particular, results suggested the importance of anaerobic degradation pathways under high-sulfate conditions with an emphasis on fumarate addition. Under low-sulfate conditions, a mixture of both aerobic and anaerobic pathways was observed but with a predominance of aerobic dioxygenases. The putative low-molecular-weight biosurfactant, lichysein, appeared to play a more important role compared to rhamnolipids. The methods used in this study-subsurface environmental samplers in combination with metagenomic sequencing of both total and translationally active metagenomes-offer a deeper and environmentally relevant perspective on community genetic potential from coal seams poised at different redox conditions broadening the understanding of degradation strategies for subsurface carbon.
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