Evidence map›Paper›PMID 35176290›Full record

ArticleJournal of molecular biology2022

Characterization of a Salmonella Transcription Factor-DNA Complex and Identification of the Inducer by Native Mass Spectrometry.

Blake E Szkoda, Angela Di Capua, Joy Shaffer, Edward J Behrman, Vicki H Wysocki, Venkat Gopalan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Expanding Native Mass Spectrometry to the Masses.Journal of the American Society for Mass Spectrometry · 2024
    Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Blake E SzkodaDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA; The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA.
Angela Di CapuaDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA; Resource for Native Mass Spectrometry-Guided Structural Biology, The Ohio State University, Columbus, OH 43210, USA.
Joy ShafferDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Edward J BehrmanDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Vicki H WysockiDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA; The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA; Resource for Native Mass Spectrometry-Guided Structural Biology, The Ohio State University, Columbus, OH 43210, USA; Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA. Electronic address: wysocki.11@osu.edu.
Venkat GopalanDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA; The Ohio State Biochemistry Program, The Ohio State University, Columbus, OH 43210, USA; Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA. Electronic address: gopalan.5@osu.edu.
The Ohio State University · US

Funding

Translational Therapeutics Research Program (TT)P30CA016058 · NCI · OHIO STATE UNIVERSITY · PI Jennifer A. Woyach · 1985 to 2026
$132.3M
Waters Select Series Cyclic IMS for P41 Native MS Resource Application to Alzheimer's DiseaseP41GM128577 · NIGMS · OHIO STATE UNIVERSITY · PI WYSOCKI, VICKI H. · 2018 to 2022
$7.6M
Salmonella-specific therapeuticsR01AI140541 · NIAID · OHIO STATE UNIVERSITY · PI AHMER, BRIAN M, GOPALAN, VENKAT · 2018 to 2021
$2.8M
Salmonella, colonization resistance, and fructose-asparagineR01AI116119 · NIAID · OHIO STATE UNIVERSITY · PI AHMER, BRIAN M, BEHRMAN, EDWARD J. · 2015 to 2019
$2.5M
Cellular, molecular, and biochemical sciences training grantT32GM086252 · NIGMS · OHIO STATE UNIVERSITY · PI JACKMAN, JANE ELIZABETH, MUSIER-FORSYTH, KARIN M · 2011 to 2020
$2.0M
15 Tesla Bruker solariX FTICR MSS10OD018507 · OD · OHIO STATE UNIVERSITY · PI WYSOCKI, VICKI H. · 2014 to 2014
$2.0M
NCI NIH HHS P30 CA016058NIAID NIH HHS R01 AI116119NIAID NIH HHS R01 AI140541NIGMS NIH HHS P41 GM128577NIGMS NIH HHS T32 GM086252NIH HHS S10 OD018507
6 · The paper itself

Abstract

FraR, a transcriptional repressor, was postulated to regulate the metabolism of the Amadori compound fructose-asparagine (F-Asn) in the foodborne pathogen Salmonella enterica. Here, the DNA- and inducer-binding affinities and stoichiometries of FraR were determined and cross-validated by electrophoretic mobility-shift assays (EMSAs) and online buffer exchange coupled to native mass spectrometry (OBE-nMS). We demonstrate the utility of OBE-nMS to characterize protein and protein-DNA complexes that are not amenable to offline exchange into volatile buffers. OBE-nMS complemented EMSAs by revealing that FraR binds to the operator DNA as a dimer and by establishing 6-phosphofructose-aspartate as the inducer that weakens DNA binding by FraR. These results provide insights into how FraR regulates the expression of F-Asn-catabolizing enzymes and add to our understanding of the intricate bacterial circuitry that dictates utilization of diverse nutrients.

Indexed as

Bacterial ProteinsSalmonella entericaTranscription FactorsAsparagineDNAFructoseMass SpectrometryAsparagineBacterial ProteinsDNAFructoseTranscription Factorsfructose-asparagine utilizationnative mass spectrometrySamonella FraRtranscriptional regulation

Identifiers

PMID35176290
PMCPMC8977229
OpenAlexW4213293860

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.