Evidence map›Paper›PMID 35175604›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast.

Jakub Sumbal, Thorarinn Gudjonsson, Gunnhildur Asta Traustadottir, Zuzana Koledova

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Jakub SumbalDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.ORCID 0000-0003-3700-4518
Thorarinn GudjonssonStem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Reykjavik, Iceland.ORCID 0000-0001-9645-9665
Gunnhildur Asta TraustadottirStem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Reykjavik, Iceland. gutra@hi.is.ORCID 0000-0001-6044-0989
Zuzana KoledovaDepartment of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. koledova@med.muni.cz.ORCID 0000-0002-9333-1399
Masaryk University · CZReykjavík University · ISUniversity of Iceland · IS

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Epithelial-stromal interactions play an essential role in regulation of mammary gland development, homeostasis, and tumorigenesis. Fibroblasts constitute a substantial proportion of mammary gland stromal cells in human breast and have been recognized for their paracrine signaling and extracellular matrix production and remodeling roles during normal breast development as well as in breast cancer. However, our current knowledge on human breast fibroblast functions is incomplete. Here we provide a detailed protocol for an organotypic human breast assay to facilitate research in the roles of human breast fibroblasts in mammary epithelial morphogenesis and early tumorigenesis.

Indexed as

Breast NeoplasmsMammary Glands, HumanAnimalsBreastEpithelial CellsFemaleFibroblastsHumansMammary Glands, AnimalParacrine CommunicationStromal Cells3D cultureBreastEpithelial-stromal interactionsFibroblastMammary glandSpheroid

Identifiers

PMID35175604
OpenAlexW4213047028

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.