Evidence map›Paper›PMID 35175493›Full record

ArticleIn vitro cellular & developmental biology. Animal2022

Osteogenic differentiation of human induced pluripotent stem cell in the presence of testosterone and 17 β-estradiol in vitro.

Reyhaneh Yarmohammadi, Marzieh Ghollasi, Fatemeh Kheirollahzadeh, Maryam Soltanyzadeh, Masoumeh Heshmati, Mohammad Amir Amirkhani

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Article in In vitro cellular & developmental biology. Animal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Reyhaneh YarmohammadiStem Cell and Regenerative Medicine Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran.
Marzieh GhollasiDepartment of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, P. O. Box, 15719-14911, Tehran, Iran. ghollasi@khu.ac.ir.
Fatemeh KheirollahzadehDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Maryam SoltanyzadehDepartment of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, P. O. Box, 15719-14911, Tehran, Iran.
Masoumeh HeshmatiDepartment of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Mohammad Amir AmirkhaniStem Cell and Regenerative Medicine Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, numerous scientific approaches have been explored to treat various diseases using stem cells. In 2006, induced pluripotent stem cell (iPSC) were introduced by Takahashi and Yamanaka and showed the potential of self-renewing and differentiation into all types of targeted cells in vitro. In this investigation, we studied the effect of testosterone (T) individually or in the presence of 17 β-estradiol (E2) on osteogenic differentiation of human iPSC (hiPSC) during 2 wk. The optimal concentrations of sex steroid hormones were examined by MTT assay and acridine orange (AO) staining. The impact of E2 and T either individually or together as a combination was examined by ALP activity; the content of total mineral calcium, by von Kossa and alizarin red staining. Additionally, the expression rate of osteogenic specific markers was studied via real-time RT-PCR and immunocytochemistry analyses at day 14 of differentiation. The obtained results illustrated that the differentiation medium supplemented with T-E2 increased not only the ALP enzyme activity and the content of calcium but also the osteogenic-related gene and protein expressions on the 14

Indexed as

Induced Pluripotent Stem CellsMesenchymal Stem CellsAnimalsCell DifferentiationCells, CulturedEstradiolHumansOsteogenesisTestosteroneEstradiolTestosteroneEstrogenHuman induced pluripotent stem cellsOsteogenic differentiationTestosterone

Identifiers

PMID35175493

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.