ArticleApplied microbiology and biotechnology2022
Biochemical characterization and cleavage pattern analysis of a novel chitosanase with cellulase activity.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Structure-based mining of a chitosanase with distinctive degradation mode and product specificity.Applied microbiology and biotechnology · 2023Article
- Insights into promiscuous chitosanases: the known and the unknown.Applied microbiology and biotechnology · 2022Review
- Biodegradation and Prospect of Polysaccharide from Crustaceans.Marine drugs · 2022Review
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Authors and funding
5 authors.
Funding
Abstract
Chitosanases are critical tools for the preparation of active oligosaccharides, whose composition is related to the cleavage pattern of the enzyme. Although numerous chitosanases have been characterized, the glycoside hydrolase (GH) family 5 chitosanases with other activities have rarely been investigated. Herein, a novel and second GH5 chitosanase OUC-Csngly from Streptomyces bacillaris was cloned and further characterized by expression in Escherichia coli BL21 (DE3). Interestingly, OUC-Csngly possessed dual chitosanase and cellulase activities. Molecular docking analysis showed that the C-2 group of sugar units affected the binding of the enzyme to oligosaccharides, which could result in different cleavage patterns toward chito-oligosaccharides (COSs) and cello-oligosaccharides. Further, we characterized OUC-Csngly's distinctive cleavage patterns toward two different types of oligosaccharides. Meanwhile, endo-type chitosanase OUC-Csngly generated (GlcN) - (GlcN)
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