ArticleFrontiers in molecular biosciences2021
Development and Application of RAA Nucleic Acid Test Strip Assay and Double RAA Gel Electrophoresis Detection Methods for ASFV and CSFV.
Article in Frontiers in molecular biosciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- Field-deployable multiplex RAA-CRISPR/Cas12a platform rapidly and simultaneously detects seven Eimeria species in chickens.Poultry science · 2026Article
- Developing a recombinase-aided amplification method combined with a lateral flow dipstick assay for rapid triplex detection of bovine coronavirus, infectious bovine rhinotracheitis virus, and bovine viral diarrhea virus.Microbiology spectrum · 2026Article
- Establishment and Evaluation of a Multicolor Latex Microsphere-Based Lateral Flow Immunoassay for the Simultaneous Detection of Antibodies Against African and Classical Swine Fever Viruses.Transboundary and emerging diseases · 2026Article
- Article
- A direct multiplex isothermal amplification-reverse dot blot hybridization system for β-thalassemia diagnosis.Annals of hematology · 2025Article
- Development of nucleic acid-based RAA test strip assay for the rapid detection of foot-and-mouth disease virus.Frontiers in veterinary science · 2025Article
- Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases.Frontiers in microbiology · 2025Review
- Using Recombinase-Aid Amplification Combined withAnimals : an open access journal from MDPI · 2024Article
- Development and Application of a TaqMan RT-qPCR for the Detection of Foot-and-Mouth Disease Virus in Pigs.Veterinary sciences · 2024Article
- Sex Identification of a Multispecies Carinatae Birds by Chicken EE0.6 Gene Using Real-Time Recombinase-Aid Amplification Assay.Ecology and evolution · 2024Article
- Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics.Veterinary research · 2024Review
- Dual Gene Detection of H5N1 Avian Influenza Virus Based on Dual RT-RPA.Molecules (Basel, Switzerland) · 2024Article
- Recombinase-aided amplification assay for rapid detection of imipenem-resistantFrontiers in cellular and infection microbiology · 2024Article
- A Recombinase-Aided Amplification Assay for the Detection ofPolish journal of microbiology · 2023Article
- Real-time recombinase-aided amplification assay for rapid amplification of the IS1081 gene of Mycobacterium tuberculosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- Development of a Novel Method for the Clinical Visualization and Rapid Identification of Multidrug-Resistant Candida auris.Microbiology spectrum · 2023Article
- Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay.Life (Basel, Switzerland) · 2023Article
- Article
- African Swine Fever Virus: A Review.Life (Basel, Switzerland) · 2022Review
- Rapid detection of influenza A viruses using a real-time reverse transcription recombinase-aided amplification assay.Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
African swine fever (ASF) is an acute, severe and hemorrhagic infectious disease caused by African swine fever virus (ASFV) infecting domestic pigs and wild boars. Since the outbreak of the disease in China in 2018, it has brought a great impact on China's pig industry. Classical swine fever (CSF) is an acute contact infectious disease of pigs caused by classical swine fever virus (CSFV) infection. Clinically, acute CSF usually shows persistent high fever, anorexia, extensive congestion and bleeding of the skin and mucosa, which are similar to ASF. It is of great significance to prevent, control and accurately detect ASF and CSF in pig farms. In this study, Recombinase aided amplification (RAA) technology combined with a nucleic acid test strip (RAA-strip) was established for simple and specific detection of ASFV/CSFV. The sensitivity and preliminary clinical application results showed that the RAA test strip established in this study could detect recombinant plasmids containing ASFV/CSFV gene fragments as low as 10
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