ArticleAmerican journal of translational research2022
Gene expression analysis reveals immune and metabolic candidate pathways in the pathogenesis of chronic otitis media.
Article in American journal of translational research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors at 2 institutions in 1 country.
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Abstract
objectivesChronic otitis media (COM) is a common disease and causes significant hearing impairment in many adults. Our study aimed to investigate the immune response in COM adult patients through RNA sequencing.
methodsIn this study, we enrolled four adult COM patients and five healthy controls (HCs) to analyze and compare the mRNA signatures in their peripheral blood mononuclear cells (PBMCs). Gene Ontology functional enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed on the differentially expressed genes between the COM patients and the HCs. Furthermore, the immune cell proportions of the two groups were estimated by CIBERSORT. The GSE125532, GSE27990, and GSE23140 gene expression profiles were also retrieved and analyzed for comparison with our results.
resultsMany immune pathways and genes were upregulated in the COM patients, including the IL-17 pathway and NLRP3. Monocytes and macrophages increased (P=0.005 and P=0.033, respectively), and the CD8+ T cells decreased (P=0.033) in the COM patients compared to the HCs. The COM patients' signatures also reflected a hypoxic state.
conclusionsOur findings emphasized the roles of several immune pathways and a hypoxic state in interpreting host responses during COM, and may enable the development of novel therapeutic tools for the disease.
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35173843PMC8829611W4213392872What OpenQuestion holds
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