ArticleScientific reports2022
Expression of genes and localization of enzymes involved in polyunsaturated fatty acid synthesis in rabbit testis and epididymis.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Alfalfa and Linseed Oil in Diet for Fattening Rabbits: Performance, Meat Quality, and Nutritional Characteristics.Animals : an open access journal from MDPI · 2025Article
- Dietary Cannabidiol Supplementation on Growth Performance, Behavior, Blood Profile, Metabolomic Analysis, and Fatty Acid Composition in Rabbits: A Multi-Disciplinary Approach to Improve Welfare and Productivity.Veterinary sciences · 2025Article
- Melatonin as a potential adjuvant to mitigate depakine‑induced testicular damage in rats through its biological features.BMC complementary medicine and therapies · 2025Article
- Gut microbiota is involved in male reproductive function: a review.Frontiers in microbiology · 2024Review
- A comprehensive review of the family of very-long-chain fatty acid elongases: structure, function, and implications in physiology and pathology.European journal of medical research · 2023Review
- Dietary supplementation with n-3 polyunsaturated fatty acids: Effects on reproductive and productive performance and meat quality in rabbit breeding.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Can Dietary n-3 Polyunsaturated Fatty Acids Affect Apelin and Resolvin in Testis and Sperm of Male Rabbits?Molecules (Basel, Switzerland) · 2023Article
- Resumption of Spermatogenesis and Fertility Post Withdrawal of Hydroxyurea Treatment.International journal of molecular sciences · 2023Article
- Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens.Frontiers in physiology · 2023Article
- Composition of Fatty Acids and Localization ofAnimals : an open access journal from MDPI · 2022Article
- Article
- Oxidation of Polyunsaturated Fatty Acids as a Promising Area of Research in Infertility.Antioxidants (Basel, Switzerland) · 2022Review
- Poultry Meat and Eggs as an Alternative Source of n-3 Long-Chain Polyunsaturated Fatty Acids for Human Nutrition.Nutrients · 2022Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The metabolism of polyunsaturated fatty acids (PUFAs) plays an important role in male reproduction. Linoleic and alpha-linolenic acids need to be provided in the diet and they are converted into long chain polyunsaturated fatty acids by steps of elongation and desaturation, exerted by elongases 2 (ELOVL2) and 5 (ELOVL5) and Δ5- (FADS1) and Δ6-desaturase (FADS2). This study aims to assess the gene expression and localization of enzymes involved in the synthesis of n-3 and n-6 long-chain PUFAs in control rabbits and those fed diets containing 10% extruded flaxseed. Enzyme and PUFA localization were assessed in the testes and epididymis by immunofluorescence. Testes showed high gene expression of FADS2, ELOVL2 and ELOVL5 and low expression of FADS1. Intermediate metabolites, enzymes and final products were differently found in Leydig, Sertoli and germinal cells. FADS2 was localized in interstitial cells and elongated spermatids; ELOVL5 in meiotic cells; FADS1 was evident in interstitial tissue, Sertoli cells and elongated spermatids; ELOVL2 in interstitial cells. Epididymal vesicles were positive for FADS1, ELOVL2 and ELOVL5 as well as docosahexaenoic, eicosapentaenoic, and arachidonic acids. This knowledge of fatty acids (FA) metabolism in spermatogenesis and the influence of diet on FA profile could help identify causes of male infertility, suggesting new personalized therapy.
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