ArticleProceedings of the National Academy of Sciences of the United States of America2022
A high-throughput technique to map cell images to cell positions using a 3D imaging flow cytometer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A high-throughput, flow cytometry approach to measure phase behavior and exchange in biomolecular condensates.Nature communications · 2026Article
- Single-pixel imaging flow cytometry for biomedical research.Inflammation and regeneration · 2025Review
- Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE).Light, science & applications · 2025Article
- Receptor-Mediated SPION Labeling of CD4Nanomaterials (Basel, Switzerland) · 2025Article
- A comprehensive update on the application of high-throughput fluorescence imaging for novel drug discovery.Expert opinion on drug discovery · 2025Review
- Highly Efficient Calibration-Free Color Compensation Algorithm for Imaging Flow Cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2025Article
- Review
- Review
- High-throughput fluorescence lifetime imaging flow cytometry.Nature communications · 2024Article
- Interpretable unsupervised learning enables accurate clustering with high-throughput imaging flow cytometry.Scientific reports · 2023Article
- Spatial multi-omics analyses of the tumor immune microenvironment.Journal of biomedical science · 2022Review
- YOLO Algorithm for Long-Term Tracking and Detection of Escherichia Coli at Different Depths of Microchannels Based on Microsphere Positioning Assistance.Sensors (Basel, Switzerland) · 2022Article
- Developments of Conventional and Microfluidic Flow Cytometry Enabling High-Throughput Characterization of Single Cells.Biosensors · 2022Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We develop a high-throughput technique to relate positions of individual cells to their three-dimensional (3D) imaging features with single-cell resolution. The technique is particularly suitable for nonadherent cells where existing spatial biology methodologies relating cell properties to their positions in a solid tissue do not apply. Our design consists of two parts, as follows: recording 3D cell images at high throughput (500 to 1,000 cells/s) using a custom 3D imaging flow cytometer (3D-IFC) and dispensing cells in a first-in-first-out (FIFO) manner using a robotic cell placement platform (CPP). To prevent errors due to violations of the FIFO principle, we invented a method that uses marker beads and DNA sequencing software to detect errors. Experiments with human cancer cell lines demonstrate the feasibility of mapping 3D side scattering and fluorescent images, as well as two-dimensional (2D) transmission images of cells to their locations on the membrane filter for around 100,000 cells in less than 10 min. While the current work uses our specially designed 3D imaging flow cytometer to produce 3D cell images, our methodology can support other imaging modalities. The technology and method form a bridge between single-cell image analysis and single-cell molecular analysis.
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