ArticleThe British journal of radiology2022
FLASH irradiation induces lower levels of DNA damage ex vivo, an effect modulated by oxygen tension, dose, and dose rate.
Article in The British journal of radiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- The FLASH effect-an evaluation of preclinical studies of ultra-high dose rate radiotherapy.Frontiers in oncology · 2024Pooled it
- Harnessing Particle Ions for FLASH Radiotherapy.Cells · 2026Review
- FLASH radiotherapy: physico-chemical considerations on ionisation tracks, radical reactions, and the role of oxygen.Radiation oncology (London, England) · 2026Review
- Harnessing the sparing effect of FLASH-RT: From phenomenon observation, radiophysical determinants to molecular mechanisms and synergistic strategies.Materials today. Bio · 2026Review
- Article
- FLASH radiotherapy enables dose escalation resulting in improved survival in an orthotopic muscle-invasive bladder cancer mouse model.The British journal of radiology · 2026Article
- Systematic tissue oxygen variation shows the modulation of murine skin radiation toxicity at ultra-high dose rates.Physics in medicine and biology · 2026Article
- Ultra-high dose rate dependent modeling of plasmid DNA damage with TOPAS-nBio.Physics in medicine and biology · 2026Article
- The Use of Synchrotron Radiation in the Medical Sciences.Photon science · 2026Article
- Oxygen Depletion in FLASH Particle Therapy: Effects of Linear Energy Transfer and Ion Track Structure.Antioxidants (Basel, Switzerland) · 2026Article
- Biological effects of conventional and ultra high dose rate radiation in human cells.Scientific reports · 2026Article
- Early molecular changes induced by FLASH irradiation in MCF10A and MDA-MB-231 breast cell lines.Frontiers in oncology · 2026Article
- FLASH Radiotherapy in Lung Cancer: Translational Mechanisms, Preclinical Evidence, and Barriers.Cureus · 2026Review
- In vitro oxygen concentration alters reactive oxygen species yields with minor plasmid DNA damage change at ultra-high-dose rate.The British journal of radiology · 2026Article
- Cytogenetic Effects of Conventional and Ultra High Dose Rates of Radiation in Human Lymphocytes: Comparative Analysis in Metaphase Chromosomes and G2-PCCs.Disaster medicine and public health preparedness · 2025Article
- Factors Influencing the Biological Effects of FLASH Irradiation.Antioxidants (Basel, Switzerland) · 2025Review
- Intermediate tissue oxygen level is required to observe murine FLASH skin sparing.bioRxiv : the preprint server for biology · 2025Article
- FLASH-induced DNA damage reduction measured in vitro correlates with effective oxygen depletion determined in silico: further support for oxygen depletion contributing to FLASH's reduced damage burden in vitro.The British journal of radiology · 2025Article
- New Approaches in Radiotherapy.Cancers · 2025Review
- Ultrahigh Dose Rate Helium Ion Beams: Minimizing Brain Tissue Damage while Preserving Tumor Control.Molecular cancer therapeutics · 2025Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveFLASH irradiation reportedly produces less normal tissue toxicity, while maintaining tumour response. To investigate oxygen's role in the 'FLASH effect', we assessed DNA damage levels following irradiation at different oxygen tensions, doses and dose rates.
methodsSamples of whole blood were irradiated (20 Gy) at various oxygen tensions (0.25-21%) with 6 MeV electrons at dose rates of either 2 kGy/s (FLASH) or 0.1 Gy/s (CONV), and subsequently with various doses (0-40 Gy) and intermediate dose rates (0.3-1000 Gy/s). DNA damage of peripheral blood lymphocytes (PBL) were assessed by the alkaline comet assay.
resultsFollowing 20 Gy irradiation, lower levels of DNA damage were induced for FLASH, the difference being significant at 0.25% (
conclusionThis study shows, using the alkaline comet assay, that lower levels of DNA damage are induced following FLASH irradiation, an effect that is modulated by the oxygen tension, and increases with the total dose and dose rate of irradiation, indicating that an oxygen related mechanism, ADVANCES IN KNOWLEDGE: This paper is first to directly show that FLASH-induced DNA damage is modulated by oxygen tension, total dose and dose rate, with FLASH inducing significantly lower levels of DNA damage for doses ≥20 Gy and dose rates ≥30 Gy/s, at 0.5% O
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