ArticleScience advances2022
In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging.
Article in Science advances, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Seeing the Chemistry of Biomolecular Condensates: In Situ Mapping of Composition and Water Content.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Resolving fluorescently labeled species using highly multiplexed spectral FLIM.Scientific reports · 2026Article
- Fluorescence-based mapping of condensate dielectric permittivity uncovers hydrophobicity-driven membrane interactions.Nature communications · 2026Article
- Biomolecular Condensates, Crowding, and Membranes: The Role of Water Revealed with Advanced Fluorescence Microscopy and Phasor Analysis.Sub-cellular biochemistry · 2026Review
- Crowding in Anhydrobiosis.Sub-cellular biochemistry · 2025Review
- Zebrafish Optical Development Requires Regulated Water Permeability by Aquaporin 0.Investigative ophthalmology & visual science · 2024Article
- Molecular Dynamics Simulations of the Eye Lens Water Channel Aquaporin 0 from Fish.The journal of physical chemistry. B · 2024Article
- Breaking Barriers: Nanomedicine-Based Drug Delivery for Cataract Treatment.International journal of nanomedicine · 2024Review
- Biomolecular condensates modulate membrane lipid packing and hydration.Nature communications · 2023Article
- Phasor plots and the future of spectral and lifetime imaging.Nature methods · 2023Article
- Adaptive Optical Two-Photon Fluorescence Microscopy Probes Cellular Organization of Ocular Lenses In Vivo.Investigative ophthalmology & visual science · 2023Article
- Stress-dependent macromolecular crowding in the mitochondrial matrix.The EMBO journal · 2023Article
- Adaptive optical two-photon fluorescence microscopy probes cellular organization of ocular lenses in vivo.bioRxiv : the preprint server for biology · 2023Article
- Methods for studying mammalian aquaporin biology.Biology methods & protocols · 2023Review
- Quantitative melanoma diagnosis using spectral phasor analysis of hyperspectral imaging from label-free slices.Frontiers in oncology · 2023Article
- Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases.Nature communications · 2022Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular crowding and water dynamics are needed to understand their roles in cellular physiology and fate determination. Macromolecular crowding in the lens is essential for normal optics, and an understanding of its regulation will help prevent cataract and presbyopia. Here, we combine the use of the nanoenvironmental sensor [6-acetyl-2-dimethylaminonaphthalene (ACDAN)] to visualize lens macromolecular crowding with in vivo studies of aquaporin 0 zebrafish mutants that disrupt its regulation. Spectral phasor analysis of ACDAN fluorescence reveals water dipolar relaxation and demonstrates that mutations in two zebrafish aquaporin 0s, Aqp0a and Aqp0b, alter water state and macromolecular crowding in living lenses. Our results provide in vivo evidence that Aqp0a promotes fluid influx in the deeper lens cortex, whereas Aqp0b facilitates fluid efflux. This evidence reveals previously unidentified spatial regulation of macromolecular crowding and spatially distinct roles for Aqp0 in the lens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.