The Cellular Behavior, Intracellular Signaling Profile and Nuclear-Targeted Potential Functions of Porcine Growth Hormone (pGH) in Swine Testicular Cells.

Yan Zhang, Qingrong Zhao, Deyi Wu, Shichun Li, Min Wu, Suo Li, Xin Zheng, Hainan Lan

Abstract read

In one paragraph

Article in Cell biochemistry and biophysics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

0.4field-weighted citation impact, top 38% of its field

1 · What the graph read from it

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0 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yan ZhangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118.

Qingrong ZhaoCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118.

Deyi WuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118.

Shichun LiThe Third Operating Room, Jilin University First Hospital, Changchun, China, 130118.

Min WuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118.

Suo LiCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118.

Xin ZhengCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118.

Hainan LanCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China, 130118. tougao@jlau.edu.cn.ORCID http://orcid.org/0000-0002-2248-8292

Jilin Agricultural University · CNJilin University · CN

Funding

National Natural Science Foundation of China 30162022

6 · The paper itself

Abstract

Porcine growth hormone (pGH) has many important biological functions and roles, and the biological activity of pGH is closely related with its cell behavior and characteristics. However, so far, the behavior of pGH in swine testicular cell remains unclear. For this, in the current work, the swine testicular cell line (ST) was used as an in vitro model, and CLSM (Confocal laser scanning microscope), IFA (Indirect immunofluorescence assay), FCM (Flow cytometry) and WB (Western-blotting) were used to explore the pGH's cell behivior and function, and the results showed that pGH and GHR could internalize into ST cell and transported to the nucleus. Furthermore, we studied the internalization kinetics of pGH and GHR on ST cell, and found that pGH and GHR internalizes into ST cell in a time-dependent manner. More importantly, we also investigated the potential molecular functions of pGH-GHR after it entered into the cell nuclei. The results indicated that nuclear-localized GHR could participate in cell proliferation by regulating the signal intensity of STAT5. In summary, our current research shows that the nuclear-localized pGH-GHR participates in the cell proliferation of ST cell, which lays a solid foundation for further research on the regulatory effect of pGH on testicular tissue.

Indexed as

Growth HormoneReceptors, SomatotropinAnimalsCell NucleusHepatocytesSignal TransductionSwineGrowth HormoneReceptors, SomatotropinNuclear localizationPorcine growth hormonePorcine growth hormone receptorProliferationSwine testicular cell

Identifiers

PMID35171434

DOI10.1016/S0021-9258(18)31758-7

OpenAlexW4213074271

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.