Evidence map›Paper›PMID 35167828›Full record

ArticleExperimental cell research2022

C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation.

Radhika Koranne, Kayla Brown, Hannah Vandenbroek, William R Taylor

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental cell research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Radhika KoranneDepartment of Biological Sciences, University of Toledo, 2801 W. Bancroft Street, MS 601, Toledo, OH, 43606, USA.
Kayla BrownDepartment of Biological Sciences, University of Toledo, 2801 W. Bancroft Street, MS 601, Toledo, OH, 43606, USA.
Hannah VandenbroekDepartment of Biological Sciences, University of Toledo, 2801 W. Bancroft Street, MS 601, Toledo, OH, 43606, USA.
William R TaylorDepartment of Biological Sciences, University of Toledo, 2801 W. Bancroft Street, MS 601, Toledo, OH, 43606, USA. Electronic address: william.taylor3@utoledo.edu.
University of Toledo · US

Funding

Regulation of Ferroptosis by the p53/CDK/Rb Axis.R15GM141712 · NIGMS · UNIVERSITY OF TOLEDO · PI TAYLOR, WILLIAM R. · 2021 to 2022
$464k
Regulation of the Mitotic Checkpoint by Gsk3R15GM120712 · NIGMS · UNIVERSITY OF TOLEDO · PI TAYLOR, WILLIAM R. · 2017 to 2017
$443k
NIGMS NIH HHS R15 GM120712NIGMS NIH HHS R15 GM141712
6 · The paper itself

Abstract

C9ORF78 is a poorly characterized protein found in diverse eukaryotes. Previous work indicated overexpression of C9ORF78 in malignant tissues indicating a possible involvement in growth regulatory pathways. Additional studies in fission yeast and humans uncover a potential function in regulating the spliceosome. In studies of GFP-tagged C9ORF78 we observed a dramatic reduction in protein abundance in cells grown to confluence and/or deprived of serum growth factors. Serum stimulation induced synchronous re-expression of the protein in HeLa cells. This effect was also observed with the endogenous protein. Overexpressing either E2F1 or N-Myc resulted in elevated C9ORF78 expression potentially explaining the serum-dependent upregulation of the protein. Immunofluorescence analysis indicates that C9ORF78 localizes to nuclei in interphase but does not appear to concentrate in speckles as would be expected for a splicing protein. Surprisingly, a subpopulation of C9ORF78 co-localizes with ACA, Mad1 and Ndc80 in mitotic cells suggesting that this protein associates with kinetochores or centromeres. Levels of C9ORF78 at the centromere/kinetochore also increased upon activation of the mitotic checkpoint. Furthermore, knocking-down C9ORF78 caused mitotic defects. These studies uncover novel mitotic function and subcellular localization of C9ORF78.

Indexed as

CentromereChromosome SegregationKinetochoresCell Cycle ProteinsHeLa CellsHumansMicrotubule-Associated ProteinsMitosisC9ORF78 protein, humanCell Cycle ProteinsMicrotubule-Associated ProteinsCentromereCheckpointKinetochoreMitosisSpliceosome

Identifiers

PMID35167828
PMCPMC12333389
OpenAlexW4210992682

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.