ArticleMethods in molecular biology (Clifton, N.J.)2022
Setting Ambient Temperature Conditions to Optimize Translation of Molecular Work from the Mouse to Human: The "Goldilocks Solution".
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 1 synthesis or guideline pooled it, 5 citations in OpenAlex.
- Under Control? Mouse Models of Heat Exposure: A Systematic Review of Experimental Design and Methodology.Acta physiologica (Oxford, England) · 2026Pooled it
- Investigation of wound healing and anti-inflammatory activity of Senna occidentalis leaf extract, and in silico screening for both activities.Pharmaceutical science advances · 2023Article
- Article
- Research Animal Behavioral Management Programs for the 21st Century.Animals : an open access journal from MDPI · 2023Review
- Deficiency of Irx5 protects mice from obesity and associated metabolic abnormalities.International journal of obesity (2005) · 2022Article
- Human total, basal and activity energy expenditures are independent of ambient environmental temperature.iScience · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temperature has a profound effect on many aspects of murine physiology. This raises the question of the best temperature at which mice should be housed to maximize the translational potential to humans. The temperatures at which mice have been routinely kept for studies of molecular physiology (20-21 °C) maximize the comfort of animal handling staff. There is a widespread movement suggesting we should perform experiments instead on mice housed at 30 °C. This often produces very different outcomes. Here we analyze the basis of this suggestion and show that while 20-21 °C is too cold, 30 °C is probably too hot. Rather we suggest an intermediate temperature "the Goldilocks solution" of 25-26 °C is probably optimal. This should be combined with providing animals with nesting material so that they can construct nests to generate microclimates that are within their own control. Providing copious nesting material has additional spin-off advantages in terms of increasing environmental enrichment. Ultimately, however, advocating a single temperature to mimic human physiology is plagued by the problem that humans vary widely in the temperature environments they experience, with consequences for human disease. Hence studying responses at a range of temperatures may provide the greatest insights and translational potential.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.