Evidence map›Paper›PMID 35167094›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Stable Isotope Tracing and Metabolomics to Study In Vivo Brown Adipose Tissue Metabolic Fluxes.

Su Myung Jung, Johnny Le, Will G Doxsey, John A Haley, Grace Park, David A Guertin, Cholsoon Jang

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Su Myung JungProgram in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA.
Johnny LeDepartment of Biological Chemistry, University of California Irvine, Irvine, CA, USA.
Will G DoxseyProgram in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA.
John A HaleyProgram in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA.
Grace ParkDepartment of Biological Chemistry, University of California Irvine, Irvine, CA, USA.
David A GuertinProgram in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA, USA. David.Guertin@umassmed.edu.
Cholsoon JangDepartment of Biological Chemistry, University of California Irvine, Irvine, CA, USA. choljang@uci.edu.

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008620 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GOLDIN, ALAN L · 1999 to 2023
$8.1M
Mechanistic Target of Rapamycin Pathways in Metabolism and Energy ExpenditureR01DK094004 · NIDDK · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GUERTIN, DAVID A · 2013 to 2023
$4.5M
Quantitative and functional analysis of brown fat nutrient fluxes in vivo and its role in organ metabolite exchangeR01DK127175 · NIDDK · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GUERTIN, DAVID A · 2021 to 2025
$2.7M
Acetyl-CoA metabolism and nutrient sensing in adipocytesR01DK116005 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI GUERTIN, DAVID A, WELLEN, KATHRYN ELAINE · 2019 to 2022
$2.0M
Elucidating dietary fructose and alcohol interactions during liver cancer developmentR01AA029124 · NIAAA · UNIVERSITY OF CALIFORNIA-IRVINE · PI JANG, CHOLSOON · 2021 to 2025
$2.0M
Elucidating the AKT-dependent and AKT-independent mechanisms of metabolic control by mTORC2R01CA196986 · NCI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GUERTIN, DAVID A · 2015 to 2019
$1.9M
NCI NIH HHS R01 CA196986NIAAA NIH HHS R01 AA029124NIDDK NIH HHS R01 DK094004NIDDK NIH HHS R01 DK116005NIDDK NIH HHS R01 DK127175NIGMS NIH HHS T32 GM008620
6 · The paper itself

Abstract

Brown adipose tissue (BAT) demonstrates extraordinary metabolic capacity. Previous research using conventional radio tracers reveals that BAT can act as a sink for a diverse menu of nutrients; still, the question of how BAT utilizes these nutrients remains unclear. Recent advances in mass spectrometry (MS) coupled to stable isotope tracing methods have greatly improved our understanding of metabolism in biology. Here, we have developed a BAT-tailored metabolomics and stable isotope tracing protocol using, as an example, the universally labeled

Indexed as

Adipose Tissue, BrownMetabolomicsCarbon IsotopesMass SpectrometryMetabolic Networks and PathwaysCarbon IsotopesBrown adipose tissueBrown fatGavageGlucoseLiquid chromatography-mass spectrometry (LC-MS)MetabolismMetabolomicsStable isotope tracingTemperature acclimation

Identifiers

PMID35167094
PMCPMC9924221

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.