Evidence map›Paper›PMID 35166972›Full record

ArticleJournal of fluorescence2022

The Fluorescent Effect of Withania Somnifera Chitosan Nanocomposite as an Effective Contrast Agent for Cancer Theragnostic: Experimental Study in Vitro.

Hala S Abuelmakarem, Mahmoud A Sliem, Jala El-Azab, Mostafa Farghaly, M A Om-Hashem, Wafaa A Ahmed

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 65% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Hala S AbuelmakaremSystems and Biomedical Engineering Department, The Higher Institute of Engineering, El Shoruk Academy, Elshorouk City, Cairo, Egypt. h.saad@sha.edu.eg.ORCID http://orcid.org/0000-0003-0663-5624
Mahmoud A SliemDepartment of Laser Applications in Metrology, Photochemistry and Agriculture (LAMPA), National Institute of Laser Enhanced Sciences (NILE), Cairo University, Giza, 12613, Egypt.
Jala El-AzabDepartment of Engineering Applications of Laser, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Giza Governorate, 12613, Egypt.
Mostafa FarghalySystems and Biomedical Engineering Department, The Higher Institute of Engineering, El Shoruk Academy, Elshorouk City, Cairo, Egypt.
M A Om-HashemSystems and Biomedical Engineering Department, The Higher Institute of Engineering, El Shoruk Academy, Elshorouk City, Cairo, Egypt.
Wafaa A AhmedCancer Biology Department, Biochemistry and Molecular Biology Unit, National Cancer Institute, Cairo University, Giza, Egypt.
Cairo University · EGEl Shorouk Academy · EG

Funding

National Cancer Institute, Cairo University 122512850
6 · The paper itself

Abstract

Nanomedicine and fluorescent optical imaging are effective in early cancer detection. The current study synthesized biocompatible nanocomposites from natural biomaterials towards inexpensive and safe cancer theragnostic. Two forms of nanocomposites were synthesized using the ionic gelation method: 1. Chitosan/ Withania Somnifera /tripolyphosphate nanocomposites, 2. Withania Somnifera/Chitosan nanocomposites. The nanocomposites were characterized by dynamic light scattering, zeta potential, and the transmission electron microscope. Fourier transform infrared spectroscopy analyzed the Withania Somnifera root water extract, Chitosan, and the synthesized nanocomposites. The cytotoxicity of the nanocomposites was investigated against the colon cancer cells (Caco2 cells) in the absence and the presence of laser (665 nm, 5 mW) irradiation. MTT assay evaluated the cytotoxicity, and Trypan blue assay assessed the cell viability. Cancerous cells were photographed under the inverted microscope in the presence and the absence of laser irradiation. Results were analyzed statistically using one-way variance (ANOVA) analysis with Bonferroni post-Hoc multiple two-group comparisons. The characterization results ensured the successful synthesis of Withania Somnifera/Chitosan nanocomposites. The results showed an increase in the cytotoxicity against colon carcinoma and a decrease in cell viability in the presence and absence of Near-infrared laser irradiation under the action of nanocomposites. The cytotoxicity of the synthesized nanocomposites increased by exposing the cells to the laser. The shining light of the nanocomposites appeared on the cells photographed under the inverted microscope. The synthesized natural nanocomposites promise systemic cytotoxicity will be efficient in molecular imaging in vivo applications.

Indexed as

ChitosanNanocompositesNeoplasmsWithaniaCaco-2 CellsContrast MediaHumansPlant ExtractsChitosanContrast MediaPlant ExtractsCancer treatmentChitosan nanoparticlesFluorescent nanoparticlesNear infra-red optical imagingWithania somnifera

Identifiers

PMID35166972
OpenAlexW4212925265

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.