ArticleeLife2022
Intravital deep-tumor single-beam 3-photon, 4-photon, and harmonic microscopy.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- Superconducting nanowire single-photon detectors for enhanced biomedical imaging.Journal of biomedical optics · 2026Article
- Third harmonic generation microscopy reveals structure and mucus dynamics in human airway epithelium models.iScience · 2026Article
- Glycocalyx micro- and nanodomains in cell-cell and cell-matrix interactions revealed by enhanced click chemistry.Nature communications · 2026Article
- Generation of In Vivo-Inspired 3D Collagen Models for Guided Tumor Invasion In Vitro.Current protocols · 2026Article
- Fast Tissue Clearing and Volume Imaging Techniques for Anatomy.Microscopy research and technique · 2025Review
- Article
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Efficient, broadly tunable, hollow-fiber source of megawatt pulses for multiphoton microscopy.Biomedical optics express · 2025Article
- Direct visualization of emergent metastatic features within an ex vivo model of the tumor microenvironment.Life science alliance · 2025Article
- AI-based hardware and software tools in microscopy to boost research in immunology and virology.Frontiers in immunology · 2025Review
- Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states.iScience · 2024Article
- Review
- Measurement of third order coherence by in situ autocorrelation for determining three-photon cross-sections.Biomedical optics express · 2024Article
- Article
- MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution.Nature communications · 2024Article
- Perfusion Window Chambers Enable Interventional Analyses of Tumor Microenvironments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Measurement of three-photon excitation cross-sections of fluorescein from 1154 nm to 1500 nm.Biomedical optics express · 2023Article
- Multidimensional Imaging of Breast Cancer.Cold Spring Harbor perspectives in medicine · 2023Review
- Metastasis from the tumor interior and necrotic core formation are regulated by breast cancer-derived angiopoietin-like 7.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 5 countries.
Funding
Abstract
Three-photon excitation has recently been demonstrated as an effective method to perform intravital microscopy in deep, previously inaccessible regions of the mouse brain. The applicability of 3-photon excitation for deep imaging of other, more heterogeneous tissue types has been much less explored. In this work, we analyze the benefit of high-pulse-energy 1 MHz pulse-repetition-rate infrared excitation near 1300 and 1700 nm for in-depth imaging of tumorous and bone tissue. We show that this excitation regime provides a more than 2-fold increased imaging depth in tumor and bone tissue compared to the illumination conditions commonly used in 2-photon excitation, due to improved excitation confinement and reduced scattering. We also show that simultaneous 3- and 4-photon processes can be effectively induced with a single laser line, enabling the combined detection of blue to far-red fluorescence together with second and third harmonic generation without chromatic aberration, at excitation intensities compatible with live tissue imaging. Finally, we analyze photoperturbation thresholds in this excitation regime and derive setpoints for safe cell imaging. Together, these results indicate that infrared high-pulse-energy low-repetition-rate excitation opens novel perspectives for intravital deep-tissue microscopy of multiple parameters in strongly scattering tissues and organs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.