ArticleThe Plant cell2022
The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 60 citations in OpenAlex.
- Single-cell insights into plant growth, adaptation, and evolution.Journal of integrative plant biology · 2026Review
- GhNAC47 activates GhSKS6-mediated cell wall remodeling to promote cotton chemical defoliation.The New phytologist · 2026Article
- Single-nucleus RNA sequencing reveals a spatiotemporal pattern of HScience China. Life sciences · 2026Article
- The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution.The Plant cell · 2026Article
- Single-nucleus sequencing and spatial metabolomics analysis reveal the regulatory mechanism of ginkgolic acid biosynthesis in the episperm ofHorticulture research · 2026Article
- Single-cell RNA-sequencing profiles reveal the developmental landscape of hawthorn leaves.BMC plant biology · 2026Article
- Integrated Single-Cell and Spatial Transcriptomics Reveal Cell-Type-Specific Immune Regulatory Networks in Maize Responding to Southern Corn Rust.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Serial Spatial Transcriptomes Reveal Regulatory Transitions in Maize Leaf Development.Plant biotechnology journal · 2026Article
- Better together: the transcription factor MUTE aggregates into nuclear condensates to regulate stomatal development in maize.The Plant cell · 2026Article
- Integrated experimental and computational workflows for single-cell transcriptomics in plants.Plant methods · 2026Article
- Esterified-pectin-coupled polar stiffening controls grass stomatal opening.Nature plants · 2026Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Unlocking grass leaf development: foundations for tunable cereal design.The New phytologist · 2025Review
- Single-cell sequencing and mass spectrometry imaging reveal the multicellular compartmentalization map of plant triterpenes: A ginsenoside in Panax ginseng example.Plant biotechnology journal · 2025Article
- Single-nucleus transcriptomics reveal the morphogenesis and artemisinin biosynthesis in Artemisia annua glandular trichomes.Nature communications · 2025Article
- The impact of asymmetrical gene expression on the development of spike morphology iniMetaOmics · 2025Article
- Review
- Identification of maize genes that condition early systemic infection of sugarcane mosaic virus through single-cell transcriptomics.Plant communications · 2025Article
- Integration of single-nuclei transcriptome and bulk RNA-seq to unravel the role of AhWRKY70 in regulating stem cell development in Arachis hypogaea L.Plant biotechnology journal · 2025Article
- ZmGCT1/2 negatively regulate drought tolerance in maize by inhibiting ZmSLAC1 to maintain guard cell turgor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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Authors and funding
28 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unique morphology of grass stomata enables rapid responses to environmental changes. Deciphering the basis for these responses is critical for improving food security. We have developed a planta platform of single-nucleus RNA-sequencing by combined fluorescence-activated nuclei flow sorting, and used it to identify cell types in mature and developing stomata from 33,098 nuclei of the maize epidermis-enriched tissues. Guard cells (GCs) and subsidiary cells (SCs) displayed differential expression of genes, besides those encoding transporters, involved in the abscisic acid, CO2, Ca2+, starch metabolism, and blue light signaling pathways, implicating coordinated signal integration in speedy stomatal responses, and of genes affecting cell wall plasticity, implying a more sophisticated relationship between GCs and SCs in stomatal development and dumbbell-shaped guard cell formation. The trajectory of stomatal development identified in young tissues, and by comparison to the bulk RNA-seq data of the MUTE defective mutant in stomatal development, confirmed known features, and shed light on key participants in stomatal development. Our study provides a valuable, comprehensive, and fundamental foundation for further insights into grass stomatal function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.