Evidence map›Paper›PMID 35166207›Full record

ArticleBiomedical journal2021

Physical attributes of salivary calcium particles and their interaction with gingival epithelium.

Hsin-Hsin Peng, Pei-Rong Huang, John D Young, David M Ojcius

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Hsin-Hsin PengCenter for Molecular and Clinical Immunology, Chang Gung University, Taoyuan, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan, Taiwan; Laboratory Animal Center, Chang Gung Memorial Hospital at LinKou, Taoyuan, Taiwan.
Pei-Rong HuangR & D Center, Cell Doctor International Co., Taipei, Taiwan.
John D YoungCenter for Molecular and Clinical Immunology, Chang Gung University, Taoyuan, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan, Taiwan; Chang Gung Immunology Consortium, Chang Gung Memorial Hospital at LinKou, Taoyuan, Taiwan; Biochemical Engineering Research Center, Ming Chi University of Technology, New Taipei, Taiwan. Electronic address: jdyoung@mail.cgu.edu.tw.
David M OjciusCenter for Molecular and Clinical Immunology, Chang Gung University, Taoyuan, Taiwan; Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan, Taiwan; Chang Gung Immunology Consortium, Chang Gung Memorial Hospital at LinKou, Taoyuan, Taiwan; Department of Biomedical Sciences, University of the Pacific, Arthur Dugoni School of Dentistry, San Francisco, CA, USA. Electronic address: dojcius@pacific.edu.
Chang Gung University · TWMing Chi University of Technology · TWUniversity of the Pacific · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe formation of dental plaque and its involvement in the pathogenesis of periodontitis is a topic of intense interest given the high prevalence of periodontitis in humans. Even though calcium-based particles play an active role in both dental plaque formation and periodontitis, few publications describe the physical-chemical properties of these particles.

methodsSaliva samples were collected from healthy volunteers. From these samples, saliva-derived particles were isolated and stained for calcium using calcein or Fluo-4. The salivary particles were also subjected to characterization by flow cytometry and immunoblotting. Internalization of calcein-labeled salivary particles by gingival epithelial cells was visualized by confocal microscopy.

resultsWe found that calcium-based salivary particles from healthy volunteers varied greatly in size but were enriched in particles of sizes at or greater than 1.5 μm. Immunoblotting analysis of the salivary particles identified several proteins including albumin, fetuin-A, and statherin, which have been found in calcium phosphate particles from other tissues or are known to modulate calcium homeostasis in saliva. In addition, calcium particles were internalized by both gingival epithelial cells and monocyte-derived macrophages.

conclusionSalivary calcium particles were enriched in the micrometer range, internalized by gingival epithelial cells, and contain albumin, fetuin-A and statherin, regulators of particle formation. These characteristics of the calcium-based salivary particles and their biological activities provide a basis for further studies to understand the molecular basis for pathogenesis of periodontitis.

Indexed as

CalciumPeriodontitisEpitheliumGingivaHumansSalivaCalciumCalciumGingival epitheliumInnate immunityParticle internalizationSalivaSalivary particles

Identifiers

PMID35166207
PMCPMC8847823
OpenAlexW3027749874

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.