Evidence map›Paper›PMID 35166073›Full record

ArticleArthritis & rheumatology (Hoboken, N.J.)2022

Characterization of Blood Mucosal-Associated Invariant T Cells in Patients With Axial Spondyloarthritis and of Resident Mucosal-Associated Invariant T Cells From the Axial Entheses of Non-Axial Spondyloarthritis Control Patients.

Nicolas Rosine, Hannah Rowe, Surya Koturan, Hanane Yahia-Cherbal, Claire Leloup, Abdulla Watad, Francis Berenbaum, Jeremie Sellam, Maxime Dougados, Vishukumar Aimanianda and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis & rheumatology (Hoboken, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 34 citations in OpenAlex.

  1. Review
  2. Article
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  4. Mucosal-Associated Invariant T Cells in Rheumatic Diseases.Arthritis & rheumatology (Hoboken, N.J.) · 2026
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  7. Article
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  11. Review
  12. Article
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  16. The bone marrow side of axial spondyloarthritis.Nature reviews. Rheumatology · 2023
    Review
  17. Review
  18. Uncovering the Underworld of Axial Spondyloarthritis.International journal of molecular sciences · 2023
    Review
  19. Review
  20. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 2 countries.

Nicolas RosineInstitut Pasteur, Université de Paris, Immunoregulation Unit, Department of Immunology, Paris, France.ORCID 0000-0003-1064-2077
Hannah RoweUniversity of Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds, UK.
Surya KoturanInstitut Pasteur, Université de Paris, Immunoregulation Unit, Department of Immunology, Paris, France.
Hanane Yahia-CherbalInstitut Pasteur, Université de Paris, Immunoregulation Unit, Department of Immunology, Paris, France.
Claire LeloupInstitut Pasteur, Université de Paris, Immunoregulation Unit, Department of Immunology, Paris, France.
Abdulla WatadUniversity of Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds, UK.
Francis BerenbaumSorbonne Université, Service de Rhumatologie, Hôpital Saint-Antoine, AP-HP, and Centre de Recherche Saint-Antoine, INSERM UMRS 938, Paris, France.
Jeremie SellamSorbonne Université, Service de Rhumatologie, Hôpital Saint-Antoine, AP-HP, and Centre de Recherche Saint-Antoine, INSERM UMRS 938, Paris, France.
Maxime DougadosINSERM Unité 1153, Clinical epidemiology and biostatistics, PRES Université Sorbonne Paris Cité, Université de Paris, Service de Rhumatologie, Hôpital Cochin Port Royal, AP-HP, and Unité Mixte AP-HP/Institut Pasteur, Institut Pasteur, Immunoregulation Unit, Paris, France.
Vishukumar AimaniandaInstitut Pasteur, Molecular Mycology Unit, CNRS UMR 2000, Paris, France.
Richard CuthbertUniversity of Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds, UK.
Charlie BridgewoodUniversity of Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds, UK.
Darren NewtonUniversity of Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds Institute of Medical Research at St James's, and St James's University Hospital, Leeds, UK.
Elisabetta BianchiInstitut Pasteur, Université de Paris, Immunoregulation Unit, Department of Immunology, and Unité Mixte AP-HP/Institut Pasteur, Institut Pasteur, Immunoregulation Unit, Paris, France.
Lars RoggeInstitut Pasteur, Université de Paris, Immunoregulation Unit, Department of Immunology, and Unité Mixte AP-HP/Institut Pasteur, Institut Pasteur, Immunoregulation Unit, Paris, France.
Dennis McGonagleUniversity of Leeds Institute of Rheumatic and Musculoskeletal Medicine, Leeds, UK.
Corinne Miceli-RichardUniversité de Paris, Service de Rhumatologie, Hôpital Cochin Port Royal, AP-HP, and Unité Mixte AP-HP/Institut Pasteur, Institut Pasteur, Immunoregulation Unit, Paris, France.
Institut Pasteur · FRUniversity of Leeds · GBInserm · FRCentre National de la Recherche Scientifique · FR

Funding

Department of Health
6 · The paper itself

Abstract

objectiveThe importance of interleukin-17A (IL-17A) in the pathogenesis of axial spondyloarthritis (SpA) has been demonstrated by the success of IL-17A blockade. However, the nature of the cell populations that produce this important proinflammatory cytokine remains poorly defined. We undertook this study to characterize the major IL-17A-producing blood cell populations in the peripheral blood of patients with axial SpA, with a focus on mucosal-associated invariant T (MAIT) cells, a population known to be capable of producing IL-17.

methodsWe evaluated IL-17A production from 5 sorted peripheral blood cell populations, namely, MAIT cells, γδ T cells, CD4+ T cells, CD8+ T cells, and neutrophils, before and after stimulation with phorbol myristate acetate, the calcium ionophore A23187, and β-1,3-glucan. Expression of IL-17A transcripts and protein were determined using nCounter and ultra-sensitive Simoa technology, respectively. MAIT cells from the axial entheses of non-axial SpA control patients (n = 5) were further characterized using flow cytometric immunophenotyping and quantitative polymerase chain reaction, and the production of IL-17 was assessed following stimulation.

resultsOn a per-cell basis, MAIT cells from peripheral blood produced the most IL-17A compared to CD4+ T cells (P < 0.01), CD8+ T cells (P < 0.0001), and γδ T cells (P < 0.0001). IL-17A was not produced by neutrophils. Gene expression analysis also revealed significantly higher expression of IL17A and IL23R in MAIT cells. Stimulation of peripheral blood MAIT cells with anti-CD3/CD28 and IL-7 and/or IL-18 induced strong expression of IL17F. MAIT cells were present in the normal, unaffected entheses of control patients who did not have axial SpA and showed elevated AHR, JAK1, STAT4, and TGFB1 transcript expression with inducible IL-17A protein. IL-18 protein expression was evident in spinal enthesis digests.

conclusionBoth peripheral blood MAIT cells and resident MAIT cells in normal axial entheses contribute to the production of IL-17 and may play important roles in the pathogenesis of axial SpA.

Indexed as

Mucosal-Associated Invariant T CellsSpondylarthritisCD8-Positive T-LymphocytesHumansInterleukin-17Interleukin-18Interleukin-17Interleukin-18

Identifiers

PMID35166073
PMCPMC9825958
OpenAlexW4213299584

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.