ArticleNature microbiology2022
Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis.
Article in Nature microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 53 citations in OpenAlex.
- Intact architectures of myophage phi92 in extended and contracted states.PLoS pathogens · 2026Article
- Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.The EMBO journal · 2026Article
- Targeted delivery of diverse biomolecules with engineered bacterial nanosyringes.Nature biotechnology · 2026Article
- Engineered Bacterial Nanosyringes Induce Transient and Controllable Hepatic Immune-Metabolic Responses.Journal of microbiology and biotechnology · 2026Article
- Stepwise firing mechanism of an extracellular contractile injection system.Nature communications · 2026Article
- Structure of a contractile injection system in Salmonella enterica subsp. salamae.Nature communications · 2026Article
- A comprehensive catalogue of receptor-binding domains in extracellular contractile injection systems.Nature communications · 2026Article
- Harnessing eCISs for precision phytomicrobiome engineering and biocontrol.FEMS microbiology reviews · 2026Review
- Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states.Nature communications · 2025Article
- The spike tip protein of bacteriophage T4.bioRxiv : the preprint server for biology · 2025Article
- Function and firing of theeLife · 2025Article
- The landscape of bacterial contractile injection systems across large-scale metagenomes.Microbiology spectrum · 2025Article
- Article
- Mechanistic Insights into the Tools for Intracellular Protein Delivery.Chem & bio engineering · 2025Review
- Article
- Review
- Decoding resistance in the age of T6SS warfare.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Thermophilic bacteria employ a contractile injection system in hot spring microbial mats.The ISME journal · 2025Article
- A hunting ground for predatory bacteria at the Zhenbei seamount in the South China Sea.ISME communications · 2025Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Contractile injection systems (CISs) are phage tail-like nanomachines, mediating bacterial cell-cell interactions as either type VI secretion systems (T6SSs) or extracellular CISs (eCISs). Bioinformatic studies uncovered a phylogenetic group of hundreds of putative CIS gene clusters that are highly diverse and widespread; however, only four systems have been characterized. Here we studied a putative CIS gene cluster in the marine bacterium Algoriphagus machipongonensis. Using an integrative approach, we show that the system is compatible with an eCIS mode of action. Our cryo-electron microscopy structure revealed several features that differ from those seen in other CISs: a 'cap adaptor' located at the distal end, a 'plug' exposed to the tube lumen, and a 'cage' formed by massive extensions of the baseplate. These elements are conserved in other CISs, and our genetic tools identified that they are required for assembly, cargo loading and function. Furthermore, our atomic model highlights specific evolutionary hotspots and will serve as a framework for understanding and re-engineering CISs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.