ArticleScientific reports2022
Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 93 citations in OpenAlex.
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- Purification and characterization of the laccase produced by the Lysinibacillus boronitolerans and its role in the decolourization of synthetic dyes.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Research Progress of Laccase in Edible and Medicinal Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- Statistical optimization and metabolite analysis of Acid Yellow 137 dye biodegradation by bacterial consortium.Scientific reports · 2026Article
- Purification and biochemical characterization of laccase from3 Biotech · 2025Article
- Characterization and Functional Analysis of a Novel Fungal Immunomodulatory Protein Gene fromInternational journal of molecular sciences · 2025Article
- The application of laccase-rich extract of spent mushroom substrates for removing lignin from jute fabric waste: a dual management approach.Scientific reports · 2025Article
- Heterologous Expression of Laccase1 fromMycobiology · 2025Article
- Production, Purification and Immobilization of Laccase fromIndian journal of microbiology · 2024Article
- Laccase Production Optimization from RecombinantMolecules (Basel, Switzerland) · 2024Article
- Optimization, purification and characterization of laccase from a new endophytic Trichoderma harzianum AUMC14897 isolated from Opuntia ficus-indica and its applications in dye decolorization and wastewater treatment.Microbial cell factories · 2024Article
- A complex metabolic network and its biomarkers regulate laccase production in white-rot fungus Cerrena unicolor 87613.Microbial cell factories · 2024Article
- Exploring laccase: a sustainable enzymatic solution for the paper recycling domain.Archives of microbiology · 2024Review
- Electricity generation and oxidoreductase potential during dye discoloration by laccase-producing Ganoderma gibbosum in fungal fuel cell.Microbial cell factories · 2023Article
- Agitation role (Dissolved Oxygen) in production of laccase from newly identified Ganoderma multistipitatum sp. nov. and its effect on mycelium morphology.BMC microbiology · 2023Article
- Influence of silicon nano-particles on Avena sativa L. to alleviate the biotic stress of Rhizoctonia solani.Scientific reports · 2023Article
- Production of a novel laccase from Ceratorhiza hydrophila and assessing its potential in natural dye fixation and cytotoxicity against tumor cells.Journal, genetic engineering & biotechnology · 2023Article
- Purification and biochemical characterization of two laccase isoenzymes isolated from Trichoderma harzianum S7113 and its application for bisphenol A degradation.Microbial cell factories · 2023Article
- Genomic analysis uncovers laccase-coding genes and biosynthetic gene clusters encoding antimicrobial compounds in laccase-producing Acinetobacter baumannii.Scientific reports · 2022Article
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2 authors at 1 institution in 1 country.
Funding
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Abstract
The aim of this work to study an efficient laccase producing fungus Ganoderma leucocontextum, which was identified by ITS regions of DNA and phylogenetic tree was constructed. This study showed the laccase first-time from G. leucocontextum by using medium containing guaiacol. The growth cultural (pH, temperature, incubation days, rpm) and nutritional (carbon and nitrogen sources) conditions were optimized, which enhanced the enzyme production up to 4.5-folds. Laccase production increased 855 U/L at 40 °C. The pH 5.0 was suitable for laccase secretion (2517 U/L) on the 7th day of incubation at 100 rpm (698.3 U/L). Glucose and sucrose were good carbon source to enhance the laccase synthesis. The 10 g/L beef (4671 U/L) and yeast extract (5776 U/L) were the best nitrogen source for laccase secretion from G. leucocontextum. The laccase was purified from the 80% ammonium sulphate precipitations of protein identified by nucleotides sequence. The molecular weight (65.0 kDa) of purified laccase was identified through SDS and native PAGE entitled as Glacc110. The Glacc110 was characterized under different parameters. It retained > 90% of its activity for 16 min incubation at 60 °C in acidic medium (pH 4.0). This enzyme exerted its optimal activity at pH 3.0 and temperature 70 °C with guaiacol substrate. The catalytic parameters K
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