Evidence map›Paper›PMID 35164064›Full record

ArticleMolecules (Basel, Switzerland)2022

Expression and Surface Display of an Acidic Cold-Active Chitosanase in

Yanhong Peng, Yaping Wang, Xiaoyan Liu, Ronghua Zhou, Xianqing Liao, Yong Min, Lixin Ma, Ying Wang, Ben Rao

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. High-efficiency patatin expression strategies inSynthetic and systems biotechnology · 2025
    Article
  3. Review
  4. Article
  5. Heterologous Expression of KetoreductaseMolecules (Basel, Switzerland) · 2024
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Yanhong PengNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Yaping WangNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Xiaoyan LiuNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Ronghua ZhouNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Xianqing LiaoNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Yong MinNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Lixin MaState Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.ORCID 0000-0002-4938-4741
Ying WangNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Ben RaoNational Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Hubei Academy of Agricultural Sciences · CN

Funding

China National Key Research and Development (R&D) Program 2021YFC2100100Hubei Provincial Central Government Guides Local Science and Technology Development Special Project 2018ZYYD015Leading Talent Project of the Hubei Academy of Agricultural Sciences L2018030the basic research union special fund project of Yunnan local undergraduate universities (part) 202001BA070001-086the National Key R&D Program of China (2017YFD0200900) Subject 2 2017YFD0200902Wuhan Science and Technology Plan Project 2018020401011305
6 · The paper itself

Abstract

Chitosanase hydrolyzes β-(1,4)-linked glycosidic bonds are used in chitosan chains to release oligosaccharide mixtures. Here, we cloned and expressed a cold-adapted chitosanase (CDA, Genbank: MW094131) using multi-copy expression plasmids (CDA1/2/3/4) in

Indexed as

Cloning, MolecularBacillusBacterial ProteinsCold TemperatureEnzyme StabilityFermentationGene ExpressionGlycoside HydrolasesPichiaRecombinant ProteinsBacterial ProteinschitosanaseGlycoside HydrolasesRecombinant Proteinschiton oligosaccharideschitosanasedisplayed enzymehigh-density fermentationmulti-copy strainsPichia pastoris

Identifiers

PMID35164064
PMCPMC8839494
OpenAlexW4210533415

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.