ArticleMolecules (Basel, Switzerland)2022
Colorimetric and Electrochemical Methods for the Detection of SARS-CoV-2 Main Protease by Peptide-Triggered Assembly of Gold Nanoparticles.
Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Review
- Review
- A Prototype Assay Multiplexing SARS-CoV-2 3CL-Protease and Angiotensin-Converting Enzyme 2 for Saliva-Based Diagnostics in COVID-19.Biosensors · 2023Article
- Empirical Optimization of Peptide Sequence and Nanoparticle Colloidal Stability: The Impact of Surface Ligands and Implications for Colorimetric Sensing.ACS applied materials & interfaces · 2023Article
- Using Nanomaterials for SARS-CoV-2 Sensing via Electrochemical Techniques.Micromachines · 2023Review
- Peptide valence-induced breaks in plasmonic coupling.Chemical science · 2023Article
- Detection of COVID-19-related biomarkers by electrochemical biosensors and potential for diagnosis, prognosis, and prediction of the course of the disease in the context of personalized medicine.Analytical and bioanalytical chemistry · 2023Review
- Peptide Amphiphile Mediated Co-assembly for Nanoplasmonic Sensing.Angewandte Chemie (International ed. in English) · 2023Article
- Proteolytic Biosensors with Functional Nanomaterials: Current Approaches and Future Challenges.Biosensors · 2023Review
- Simple Method for Optical Detection and Characterization of Surface Agents on Conjugated Gold Nanoparticles.Plasmonics (Norwell, Mass.) · 2023Article
- Recent Advances in Colorimetric Sensors Based on Gold Nanoparticles for Pathogen Detection.Biosensors · 2022Review
- Spacer Matters: All-Peptide-Based Ligand for Promoting Interfacial Proteolysis and Plasmonic Coupling.Nano letters · 2022Article
- Functional Nanomaterials: From Structures to Biomedical Applications.Molecules (Basel, Switzerland) · 2022Article
- A Self-Immolative Fluorescent Probe for Selective Detection of SARS-CoV-2 Main Protease.Analytical chemistry · 2022Article
- The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review.RSC advances · 2022Review
- Nanoscale Technologies in the Fight against COVID-19: From Innovative Nanomaterials to Computer-Aided Discovery of Potential Antiviral Plant-Derived Drugs.Biomolecules · 2022Review
- Gold nanoparticle-based optical nanosensors for food and health safety monitoring: recent advances and future perspectives.RSC advances · 2022Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease (Mpro) has been regarded as one of the ideal targets for the development of antiviral drugs. The currently used methods for the probing of Mpro activity and the screening of its inhibitors require the use of a double-labeled peptide substrate. In this work, we suggested that the label-free peptide substrate could induce the aggregation of AuNPs through the electrostatic interactions, and the cleavage of the peptide by the Mpro inhibited the aggregation of AuNPs. This fact allowed for the visual analysis of Mpro activity by observing the color change of the AuNPs suspension. Furthermore, the co-assembly of AuNPs and peptide was achieved on the peptide-covered electrode surface. Cleavage of the peptide substrate by the Mpro limited the formation of AuNPs/peptide assembles, thus allowing for the development of a simple and sensitive electrochemical method for Mpro detection in serum samples. The change of the electrochemical signal was easily monitored by electrochemical impedance spectroscopy (EIS). The detection limits of the colorimetric and electrochemical methods are 10 and 0.1 pM, respectively. This work should be valuable for the development of effective antiviral drugs and the design of novel optical and electrical biosensors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.