Evidence map›Paper›PMID 35163644›Full record

ArticleInternational journal of molecular sciences2022

GFP-Margatoxin, a Genetically Encoded Fluorescent Ligand to Probe Affinity of Kv1.3 Channel Blockers.

Kristina R Denisova, Nikita A Orlov, Sergey A Yakimov, Elena A Kryukova, Dmitry A Dolgikh, Mikhail P Kirpichnikov, Alexey V Feofanov, Oksana V Nekrasova

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Kristina R DenisovaFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
Nikita A OrlovFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
Sergey A YakimovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
Elena A KryukovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
Dmitry A DolgikhFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.ORCID 0000-0002-9880-7202
Mikhail P KirpichnikovFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.
Alexey V FeofanovFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.ORCID 0000-0002-1596-9506
Oksana V NekrasovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.ORCID 0000-0002-7216-1618
Lomonosov Moscow State University · RUInstitute of Bioorganic Chemistry · RU

Funding

Ministry of Science and Higher Education of Russia Research Project N 075-15-2020-795, local identifier 13.1902.21.0027
6 · The paper itself

Abstract

Peptide pore blockers and their fluorescent derivatives are useful molecular probes to study the structure and functions of the voltage-gated potassium Kv1.3 channel, which is considered as a pharmacological target in the treatment of autoimmune and neurological disorders. We present Kv1.3 fluorescent ligand, GFP-MgTx, constructed on the basis of green fluorescent protein (GFP) and margatoxin (MgTx), the peptide, which is widely used in physiological studies of Kv1.3. Expression of the fluorescent ligand in

Indexed as

Kv1.3 Potassium ChannelBinding SitesGreen Fluorescent ProteinsHumansLigandsPeptidesPotassium Channel BlockersProtein BindingGreen Fluorescent ProteinsKCNA3 protein, humanKv1.3 Potassium ChannelLigandsPeptidesPotassium Channel Blockersaffinityfluorescent ligandGFPKv1.3 channelmargatoxinneurological disorderspore blocker

Identifiers

PMID35163644
PMCPMC8835862
OpenAlexW4210352871

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.