Evidence map›Paper›PMID 35163529›Full record

ArticleInternational journal of molecular sciences2022

Activatable Peptides for Rapid and Simple Visualization of Protease Activity Secreted in Living Cells.

Gae-Baik Kim, Jeong Min Lee, Duc Long Nguyen, Joonseok Lee, Young-Pil Kim

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Gae-Baik KimDepartment of Life Science, Hanyang University, Seoul 04763, Korea.
Jeong Min LeeDepartment of Life Science, Hanyang University, Seoul 04763, Korea.
Duc Long NguyenDepartment of Life Science, Hanyang University, Seoul 04763, Korea.ORCID 0000-0003-3588-2931
Joonseok LeeDepartment of Chemistry, Hanyang University, Seoul 04763, Korea.ORCID 0000-0001-9551-6402
Young-Pil KimDepartment of Life Science, Hanyang University, Seoul 04763, Korea.ORCID 0000-0001-7234-1320
Hanyang University · KR

Funding

Korea Institute of Science and Technology 2Z06270-20-137National Research Foundation of Korea . 2019R1A2C2004858 and 2020R1A6A1A06046728
6 · The paper itself

Abstract

Activity-based monitoring of cell-secreted proteases has gained significant interest due to the implication of these substances in diverse cellular functions. Here, we demonstrated a cell-based method of monitoring protease activity using fluorescent cell-permeable peptides. The activatable peptide consists of anionic (EEEE), cleavable, and cationic sequences (RRRR) that enable intracellular delivery by matrix metalloproteinase-2 (MMP2), which is secreted by living cancer cells. Compared to HT-29 cells (MMP2-negative), HT-1080 cells (MMP2-positive) showed a strong fluorescence response to the short fluorescent peptide via cell-secreted protease activation. Our approach is expected to find applications for the rapid visualization of protease activity in living cells.

Indexed as

Cell Line, TumorHT29 CellsHumansMatrix Metalloproteinase 2NeoplasmsOptical ImagingPeptidesProteolysisMatrix Metalloproteinase 2MMP2 protein, humanPeptidesactivatable peptidescancer cellsfluorescent imagingMMP2proteases

Identifiers

PMID35163529
PMCPMC8836073
OpenAlexW4210648946

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.