Evidence map›Paper›PMID 35163230›Full record

ArticleInternational journal of molecular sciences2022

Native Liquid Chromatography and Mass Spectrometry to Structurally and Functionally Characterize Endo-Xylanase Proteoforms.

Guusje van Schaick, Nadi El Hajjouti, Simone Nicolardi, Joost den Hartog, Romana Jansen, Rob van der Hoeven, Wim Bijleveld, Nicolas Abello, Manfred Wuhrer, Maurien M A Olsthoorn and 1 more

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Guusje van SchaickCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.ORCID 0000-0001-8320-4460
Nadi El HajjoutiCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.ORCID 0000-0002-4729-1488
Simone NicolardiCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
Joost den HartogCenter for Analytical Innovation, DSM, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Romana JansenCenter for Analytical Innovation, DSM, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Rob van der HoevenCenter for Analytical Innovation, DSM, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Wim BijleveldCenter for Analytical Innovation, DSM, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Nicolas AbelloCenter for Analytical Innovation, DSM, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Manfred WuhrerCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.ORCID 0000-0002-0814-4995
Maurien M A OlsthoornCenter for Analytical Innovation, DSM, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands.
Elena Domínguez-VegaCenter for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
DSM (Netherlands) · NLLeiden University Medical Center · NL

Funding

Dutch Research Council 731.017.202
6 · The paper itself

Abstract

Xylanases are of great value in various industries, including paper, food, and biorefinery. Due to their biotechnological production, these enzymes can contain a variety of post-translational modifications, which may have a profound effect on protein function. Understanding the structure-function relationship can guide the development of products with optimal performance. We have developed a workflow for the structural and functional characterization of an endo-1,4-β-xylanase (ENDO-I) produced by

Indexed as

Aspergillus nigerChromatography, GelChromatography, Ion ExchangeChromatography, LiquidEndo-1,4-beta XylanasesGlycosylationMass SpectrometryModels, MolecularProtein Processing, Post-TranslationalProteinsStructure-Activity RelationshipEndo-1,4-beta XylanasesProteinsboronate affinity chromatographyendo-1,4-β-xylanaseglycationion exchange chromatographymass spectrometrynative protein analysissize exclusion chromatography

Identifiers

PMID35163230
PMCPMC8835838
OpenAlexW4210476254

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.